ZFIN ID: ZDB-FIG-111115-13
O'Brien et al., 2011 - Wt1a, Foxc1a, and the Notch mediator Rbpj physically interact and regulate the formation of podocytes in zebrafish. Developmental Biology   358(2):318-30 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 14-19 somites to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 14-19 somites to Long-pec

Fig. 3

Analysis of podocyte, neck and interrenal development by in situ hybridization following morpholino knockdown of wt1a, foxc1a and/or rbpj. Each transcription factor was knocked down either by itself or in combination with each of the other factors as indicated. The expression analysis was carried out at 15 somites, 24 hpf, 36 hpf and 48 hpf. wt1b+ = podocytes; cdh17+ = non-podocyte renal epithelia; slc20a1a+ = first proximal tubule segment; nephrin+ = mature podocyte; podocalyxin+ = mature podocyte; nr5a1a+ = interrenal cells; pax2a+ = podocytes and neck segment (15 somite), neck segment (48 hpf). Analysis of hey1 expression by in situ hybridization was performed at 15 somites under single and double knockdown conditions. Arrows point to podocyte expression of hey1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cdh17 TU standard conditions Prim-5 pronephric duct ISH
TU + MO1-foxc1a standard conditions Prim-5 pronephric duct ISH
TU + MO1-foxc1a + MO3-wt1a standard conditions Prim-5 pronephric duct ISH
TU + MO1-foxc1a + MO4-rbpja,rbpjb standard conditions Prim-5 pronephric duct ISH
TU + MO3-wt1a standard conditions Prim-5 pronephric duct ISH
TU + MO3-wt1a + MO4-rbpja,rbpjb standard conditions Prim-5 pronephric duct ISH
TU + MO4-rbpja,rbpjb standard conditions Prim-5 pronephric duct ISH
hey1 TU standard conditions 14-19 somites pronephric podocyte ISH
14-19 somites somite ISH
TU + MO1-foxc1a standard conditions 14-19 somites pronephric podocyte ISH
14-19 somites somite ISH
TU + MO1-foxc1a + MO3-wt1a standard conditions 14-19 somites somite ISH
TU + MO1-foxc1a + MO4-rbpja,rbpjb standard conditions 14-19 somites somite ISH
TU + MO3-wt1a standard conditions 14-19 somites pronephric podocyte ISH
14-19 somites somite ISH
TU + MO3-wt1a + MO4-rbpja,rbpjb standard conditions 14-19 somites somite ISH
TU + MO4-rbpja,rbpjb standard conditions 14-19 somites pronephric podocyte ISH
14-19 somites somite ISH
nphs1 TU standard conditions Prim-25 pronephric podocyte ISH
TU + MO1-foxc1a standard conditions Prim-25 pronephric podocyte ISH
TU + MO1-foxc1a + MO3-wt1a standard conditions Prim-25 Not Detected pronephric podocyte ISH
TU + MO1-foxc1a + MO4-rbpja,rbpjb standard conditions Prim-25 Not Detected pronephric podocyte ISH
TU + MO3-wt1a standard conditions Prim-25 Not Detected pronephric podocyte ISH
TU + MO3-wt1a + MO4-rbpja,rbpjb standard conditions Prim-25 Not Detected pronephric podocyte ISH
TU + MO4-rbpja,rbpjb standard conditions Prim-25 pronephric podocyte ISH
nr5a1a TU standard conditions Prim-5 interrenal primordium ISH
TU + MO1-foxc1a standard conditions Prim-5 interrenal primordium ISH
TU + MO1-foxc1a + MO3-wt1a standard conditions Prim-5 Not Detected interrenal primordium ISH
TU + MO1-foxc1a + MO4-rbpja,rbpjb standard conditions Prim-5 interrenal primordium ISH
TU + MO3-wt1a standard conditions Prim-5 interrenal primordium ISH
TU + MO3-wt1a + MO4-rbpja,rbpjb standard conditions Prim-5 interrenal primordium ISH
TU + MO4-rbpja,rbpjb standard conditions Prim-5 interrenal primordium ISH
pax2a TU standard conditions 14-19 somites pronephric glomerulus ISH
14-19 somites pronephric tubule ISH
Long-pec pronephric glomerulus ISH
Long-pec pronephric tubule ISH
TU + MO1-foxc1a standard conditions 14-19 somites pronephric glomerulus ISH
14-19 somites pronephric tubule ISH
Long-pec pronephric glomerulus ISH
Long-pec pronephric tubule ISH
TU + MO1-foxc1a + MO3-wt1a standard conditions 14-19 somites pronephric glomerulus ISH
14-19 somites pronephric tubule ISH
Long-pec Not Detected pronephric glomerulus ISH
TU + MO1-foxc1a + MO4-rbpja,rbpjb standard conditions 14-19 somites pronephric glomerulus ISH
14-19 somites pronephric tubule ISH
Long-pec pronephric glomerulus ISH
Long-pec pronephric tubule ISH
TU + MO3-wt1a standard conditions 14-19 somites pronephric glomerulus ISH
14-19 somites pronephric tubule ISH
Long-pec pronephric glomerulus ISH
Long-pec pronephric tubule ISH
TU + MO3-wt1a + MO4-rbpja,rbpjb standard conditions 14-19 somites pronephric glomerulus ISH
14-19 somites pronephric tubule ISH
Long-pec Not Detected pronephric glomerulus ISH
Long-pec Not Detected pronephric tubule ISH
TU + MO4-rbpja,rbpjb standard conditions 14-19 somites pronephric glomerulus ISH
14-19 somites pronephric tubule ISH
Long-pec pronephric glomerulus ISH
Long-pec pronephric tubule ISH
podxl TU standard conditions Prim-25 pronephric podocyte ISH
TU + MO1-foxc1a standard conditions Prim-25 pronephric podocyte ISH
TU + MO1-foxc1a + MO3-wt1a standard conditions Prim-25 mesoderm ISH
TU + MO1-foxc1a + MO4-rbpja,rbpjb standard conditions Prim-25 mesoderm ISH
TU + MO3-wt1a standard conditions Prim-25 mesoderm ISH
TU + MO3-wt1a + MO4-rbpja,rbpjb standard conditions Prim-25 mesoderm ISH
TU + MO4-rbpja,rbpjb standard conditions Prim-25 pronephric podocyte ISH
slc20a1a TU standard conditions Prim-5 pronephric proximal straight tubule ISH
TU + MO1-foxc1a standard conditions Prim-5 pronephric proximal straight tubule ISH
TU + MO1-foxc1a + MO3-wt1a standard conditions Prim-5 pronephric proximal straight tubule ISH
TU + MO1-foxc1a + MO4-rbpja,rbpjb standard conditions Prim-5 pronephric proximal straight tubule ISH
TU + MO3-wt1a standard conditions Prim-5 pronephric proximal straight tubule ISH
TU + MO3-wt1a + MO4-rbpja,rbpjb standard conditions Prim-5 pronephric proximal straight tubule ISH
TU + MO4-rbpja,rbpjb standard conditions Prim-5 pronephric proximal straight tubule ISH
wt1b TU standard conditions 14-19 somites pronephric podocyte ISH
Prim-5 pronephric podocyte ISH
Prim-25 pronephric podocyte ISH
TU + MO1-foxc1a standard conditions 14-19 somites pronephric podocyte ISH
Prim-5 pronephric podocyte ISH
Prim-25 pronephric podocyte ISH
TU + MO1-foxc1a + MO3-wt1a standard conditions 14-19 somites Not Detected pronephric podocyte ISH
Prim-5 Not Detected pronephric podocyte ISH
Prim-25 Not Detected pronephric podocyte ISH
TU + MO1-foxc1a + MO4-rbpja,rbpjb standard conditions 14-19 somites Not Detected pronephric podocyte ISH
Prim-5 Not Detected pronephric podocyte ISH
Prim-25 Not Detected pronephric podocyte ISH
TU + MO3-wt1a standard conditions 14-19 somites pronephric podocyte ISH
Prim-5 pronephric podocyte ISH
Prim-25 pronephric podocyte ISH
TU + MO3-wt1a + MO4-rbpja,rbpjb standard conditions 14-19 somites Not Detected pronephric podocyte ISH
Prim-5 Not Detected pronephric podocyte ISH
Prim-25 Not Detected pronephric podocyte ISH
TU + MO4-rbpja,rbpjb standard conditions 14-19 somites pronephric podocyte ISH
Prim-5 pronephric podocyte ISH
Prim-25 pronephric podocyte ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO1-foxc1a standard conditions 14-19 somites apoptotic process process quality, normal
14-19 somites pronephric duct present, normal
14-19 somites pronephric podocyte decreased amount, abnormal
Prim-5 interrenal primordium morphology, normal
Prim-5 pronephric podocyte decreased amount, abnormal
Prim-5 pronephric proximal convoluted tubule morphology, normal
Prim-25 pronephric podocyte decreased amount, abnormal
Prim-25 pronephric proximal convoluted tubule morphology, normal
Long-pec pronephric tubule present, normal
TU + MO1-foxc1a + MO3-wt1a standard conditions 14-19 somites apoptotic process process quality, normal
14-19 somites pronephric duct present, normal
14-19 somites pronephric podocyte decreased amount, abnormal
Prim-5 interrenal primordium absent, abnormal
Prim-5 pronephric podocyte decreased amount, abnormal
Prim-5 pronephric proximal convoluted tubule morphology, normal
Prim-25 pronephric podocyte decreased amount, abnormal
Long-pec interrenal primordium absent, abnormal
Long-pec pronephric tubule absent, abnormal
TU + MO1-foxc1a + MO4-rbpja,rbpjb standard conditions 14-19 somites apoptotic process process quality, normal
14-19 somites pronephric duct present, normal
14-19 somites pronephric podocyte decreased amount, abnormal
Prim-5 interrenal primordium increased size, abnormal
Prim-5 pronephric podocyte decreased amount, abnormal
Prim-5 pronephric proximal convoluted tubule morphology, normal
Prim-25 pronephric podocyte decreased amount, abnormal
Long-pec pronephric tubule morphology, abnormal
TU + MO3-wt1a standard conditions 14-19 somites apoptotic process process quality, normal
14-19 somites pronephric duct present, normal
14-19 somites pronephric podocyte decreased amount, abnormal
Prim-5 interrenal primordium increased size, abnormal
Prim-5 pronephric podocyte decreased amount, abnormal
Prim-5 pronephric proximal convoluted tubule morphology, normal
Prim-5 pronephric tubule morphology, normal
Prim-25 pronephric podocyte decreased amount, abnormal
Long-pec pronephric tubule morphology, normal
TU + MO3-wt1a + MO4-rbpja,rbpjb standard conditions 14-19 somites apoptotic process process quality, normal
14-19 somites pronephric duct present, normal
14-19 somites pronephric podocyte decreased amount, abnormal
Prim-5 interrenal primordium increased size, abnormal
Prim-5 pronephric podocyte decreased amount, abnormal
Prim-5 pronephric proximal convoluted tubule morphology, normal
Prim-25 pronephric podocyte decreased amount, abnormal
Long-pec pronephric tubule absent, abnormal
TU + MO4-rbpja,rbpjb standard conditions 14-19 somites apoptotic process process quality, normal
14-19 somites pronephric duct present, normal
14-19 somites pronephric podocyte decreased amount, abnormal
Prim-5 interrenal primordium increased size, abnormal
Prim-5 pronephric podocyte decreased amount, abnormal
Prim-5 pronephric proximal convoluted tubule morphology, normal
Prim-5 pronephric tubule morphology, abnormal
Prim-25 pronephric podocyte decreased amount, abnormal
Long-pec pronephric tubule present, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 358(2), O'Brien, L.L., Grimaldi, M., Kostun, Z., Wingert, R.A., Selleck, R., and Davidson, A.J., Wt1a, Foxc1a, and the Notch mediator Rbpj physically interact and regulate the formation of podocytes in zebrafish, 318-30, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.