Fig. S6

Fig. S6 FoxA3 morpholinos efficiently block translation of FoxA3-GFP fusion protein. (A) Sequence of the 5′UTR and of the region coding for the Nt part of the FoxA3 protein showing the position of FoxA3 morpholinos, MO-γ1 (that covers the initiation of translation) and MO- γ3 (two nucleotides upstream of MO- γ1 in the 5′UTR part of the RNA). (B) Injection of 50 pg of RNA coding for a FoxA3-GFP fusion protein at the 4 cell stage results in embryo displaying a strong fluorescence in the nucleus of one quarter of the cells. (C) Injection of 8 ng of both MO- γ1 and MO- γ3 as well as (D) 8 ng of MO- γ1 or (E) 8 ng of MO- γ3 abolish the translation of FoxA3-GFP fusion protein (central panels in C–E compared to right panel in A). Morpholinos have been injected with a RNA coding for a RFP used as control to show that all embryos observed are morphants (right panels in C–E).