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ZFIN ID: ZDB-FIG-110105-13
Labalette et al., 2011 - Hindbrain patterning requires fine-tuning of early krox20 transcription by Sprouty 4. Development (Cambridge, England)   138(2):317-326 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-4 somites to 5-9 somites

Fig. 1 Spry gene expression in the early zebrafish hindbrain. (A-L) In situ hybridisations were performed with spry1 (A,D,G,J), spry2 (B,E,H,K) and spry4 (C,F,I,L) probes (blue) at the indicated somite (s) or epiboly (%) stages, shown as lateral views with anterior to the left (A-C,G-I) and flat-mounts with anterior at the top (D-F) or left (inset in I and J-L). Where indicated (D-F,J-L), double in situ hybridisation was performed with a krox20 probe (red) to allow precise localisation of r3 and r5. The inset in F shows the spry4 pattern without krox20 labelling. hb, hindbrain; tb, tailbud; tl, telencephalon; MHB, midbrain-hindbrain boundary.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
egr2b WT standard conditions 1-4 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
spry1 WT standard conditions Bud hindbrain neural keel ISH
1-4 somites presumptive rhombomere 1 ISH
1-4 somites presumptive rhombomere 2 ISH
1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 4 ISH
1-4 somites presumptive rhombomere 5 ISH
1-4 somites presumptive rhombomere 6 ISH
5-9 somites midbrain hindbrain boundary neural keel ISH
5-9 somites presumptive rhombomere 1 ISH
5-9 somites presumptive rhombomere 2 ISH
5-9 somites presumptive rhombomere 4 ISH
spry2 WT standard conditions Bud hindbrain neural keel ISH
1-4 somites presumptive rhombomere 1 ISH
1-4 somites presumptive rhombomere 2 ISH
1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 4 ISH
5-9 somites midbrain hindbrain boundary neural keel ISH
5-9 somites presumptive rhombomere 1 ISH
5-9 somites presumptive rhombomere 2 ISH
5-9 somites presumptive rhombomere 4 ISH
spry4 WT standard conditions Bud hindbrain neural keel ISH
1-4 somites presumptive rhombomere 2 ISH
1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 4 ISH
1-4 somites presumptive rhombomere 5 ISH
5-9 somites midbrain hindbrain boundary neural keel ISH
5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development