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Fig. 1 Spry gene expression in the early zebrafish hindbrain. (A-L) In situ hybridisations were performed with spry1 (A,D,G,J), spry2 (B,E,H,K) and spry4 (C,F,I,L) probes (blue) at the indicated somite (s) or epiboly (%) stages, shown as lateral views with anterior to the left (A-C,G-I) and flat-mounts with anterior at the top (D-F) or left (inset in I and J-L). Where indicated (D-F,J-L), double in situ hybridisation was performed with a krox20 probe (red) to allow precise localisation of r3 and r5. The inset in F shows the spry4 pattern without krox20 labelling. hb, hindbrain; tb, tailbud; tl, telencephalon; MHB, midbrain-hindbrain boundary.