FIGURE

Fig. 2

ID

ZDB-FIG-110105-14

Publication

Labalette et al., 2011 - Hindbrain patterning requires fine-tuning of early krox20 transcription by Sprouty 4

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Fig. 2

Spry4 loss-of-function results in hindbrain patterning defects. (A-G) Zebrafish embryos injected with either control morpholino (Ctrlmo) (A,F), Spry1mo (B), Spry2mo (C), Spry4mo (D,G) or both Spry1mo and Spry2mo (E) were collected at the 10-somite (A-E) or 12-somite (F,G) stage and subjected to in situ hybridisation for krox20 and her5, a marker of the MHB (A-E, both purple), or for krox20 (red), her5 and hoxb1a (purple) (F,G). Embryos are flat-mounted with anterior to the left. (H,I) Quantitative evaluation of rhombomere areas. Normalised areas were obtained by dividing each rhombomere area by one fifth of the area of the neural plate from r1 to r5. ns, not significant (P>0.05); ^*, P<0.004; ^**, P<0.0001; Student′s t-test. Errors bars indicate s.e.m.

Expression Data

Genes:	egr2b her5 hoxb1a
Fish:	WT WT + MO1-spry1 WT + MO1-spry2 WT + MO3-spry4 WT + MO1-spry1 + MO1-spry2
Knockdown Reagents:	MO1-spry1 MO1-spry2 MO3-spry4
Anatomical Terms:	midbrain hindbrain boundary neural rod rhombomere 3 rhombomere 4 rhombomere 5
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-spry2 WT + MO3-spry4 WT + MO1-spry1 + MO1-spry2
Knockdown Reagents:	MO1-spry1 MO1-spry2 MO3-spry4
Observed In:	neural plate rhombomere 3 rhombomere 4 rhombomere 5 rhombomere formation
Stage:	10-13 somites

Phenotype Detail

Acknowledgments

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