FIGURE

Fig. 3

ID

ZDB-FIG-110105-15

Publication

Labalette et al., 2011 - Hindbrain patterning requires fine-tuning of early krox20 transcription by Sprouty 4

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Fig. 3

Spry4 controls the onset of krox20 expression. (A-C) Zebrafish embryos injected with either control morpholino (Ctrlmo) (A), Spry4mo (B) or Spry2mo (C) were collected at 100% epiboly and subjected to in situ hybridisation with krox20 and ntl probes (purple). Arrows in B indicate krox20 expression in a few r5 cells. The insets show tailbud views of the embryos, allowing determination of the developmental stage by evaluation of the closure of the tailbud, as revealed by ntl expression. (D-G) Distribution of embryos showing either no krox20 expression, limited expression in r3 or expression in both r3 and r5 at 90, 95 or 100% epiboly or at 10.25 hours post-fertilisation (hpf). ns, not significant; ^*, P<0.05; ^**, P<0.0001; χ²-test.

Expression Data

Genes:	egr2b tbxta
Fish:	WT WT + MO1-spry2 WT + MO3-spry4
Knockdown Reagents:	MO1-spry2 MO3-spry4
Anatomical Terms:	notochord presumptive rhombomere 3 presumptive rhombomere 5
Stage:	Bud

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-spry4
Knockdown Reagent:	MO3-spry4
Observed In:	rhombomere 5 rhombomere formation
Stage:	Bud

Phenotype Detail

Acknowledgments

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