FIGURE

Fig. S5

ID

ZDB-FIG-101129-20

Publication

Baskin et al., 2010 - Visualizing enveloping layer glycans during zebrafish early embryogenesis

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Fig. S5

In vivo imaging of glycans during epiboly using microinjection of GalNAz and detection with copper-free click chemistry. Zebrafish embryos were microinjected with 25 pmol of GalNAz (top) or no sugar (bottom), along with the tracer dye rhodamine-dextran, and allowed to develop to 50% epiboly (5.5 hpf, 1st and 4th rows), 65% epiboly (7 hpf, 2nd and 5th rows), or 95% epiboly (9.5 hpf, 3rd and 6th rows). Embryos were then reacted with DIFO-488 (100 μM, 1 h) and imaged by confocal microscopy. Shown are maximum intensity z-projection fluorescence images and corresponding brightfield images. Green, DIFO-488; red, rhodaminedextran. Scale bar: 200 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA