FIGURE

Fig. 1

ID

ZDB-FIG-101129-12

Publication

Baskin et al., 2010 - Visualizing enveloping layer glycans during zebrafish early embryogenesis

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Fig. 1

In vivo imaging of glycans in early zebrafish embryos using microinjection of azidosugars and detection with copper-free click chemistry. (A) Metabolic labeling of mucin-type O-glycans with azidosugars via the GalNAc salvage pathway. The enzymatic transformations shown are catalyzed by (i) nonspecific esterases, (ii) GalNAc-1-phosphate kinase, (iii) UDP-GalNAc pyrophosphorylase, and (iv) ppGalNAcTs and other glycosyltransferases. (B) Two-step strategy for imaging glycans in vivo. (C and D) Zebrafish embryos were microinjected with UDP-GalNAz (C, top), GalNAz (D, top), UDP-GalNAc (C, bottom), or no sugar (D, bottom), along with the tracer dye rhodamine-dextran, allowed to develop to 7 hpf, reacted with DIFO-488 (100 μM, 1 h), and imaged by confocal microscopy. Shown are maximum intensity z-projection images. Green, DIFO-488; red, rhodamine-dextran. (Scale bar: 200 μm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA