FIGURE

Fig. S10

ID

ZDB-FIG-101129-25

Publication

Baskin et al., 2010 - Visualizing enveloping layer glycans during zebrafish early embryogenesis

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Fig. S10

Multicolor imaging of time-resolved populations of GalNAz-labeled glycans. Zebrafish embryos were microinjected with 25 pmol of GalNAz or no sugar and allowed to develop to 9 hpf, at which point they were reacted with DIFO-488 (100 μM, 1 h). The embryos were then allowed to further develop for 2 h (A) or 12 h (B), at which point they were reacted with DIFO-555 (100 μM, 1 h) and then imaged by confocal microscopy. Shown are maximum intensity z-projection fluorescence images of surface epithelial cells and corresponding brightfield images. Green, DIFO-488; red, DIFO-555. Scale bars: 200 μm (1st, 2nd, 5th, and 6th rows), 10 μm (3rd, 4th, 7th, and 8th rows).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA