FIGURE

Fig. S11

ID

ZDB-FIG-101129-26

Publication

Baskin et al., 2010 - Visualizing enveloping layer glycans during zebrafish early embryogenesis

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Fig. S11

Imaging of sialylated glycans via either microinjection of ManNAz or treatment with sodium periodate. (A) Schematic depicting metabolic approach for imaging of sialylated glycans. (B) Zebrafish embryos were microinjected with 25 pmol of ManNAz or no sugar, allowed to develop to 8 hpf, reacted with DIFO-488 (100 μM, 1 h), and imaged by confocal microscopy. Shown are maximum intensity z-projection fluorescence images and corresponding brightfield images. Green, DIFO-488; red, rhodamine-dextran. (C) Zebrafish embryos (6 hpf) were bathed in NaIO4 (500 μM, 30 min, top row) or no reagent (bottom row), reacted with AO-488 (100 μM, 1 h, pH 6.7), and imaged by confocal microscopy. Shown are maximum intensity z-projection images of AO-488 fluorescence and corresponding brightfield images. Scale bars: 200 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA