FIGURE

Fig. S2

ID

ZDB-FIG-101129-17

Publication

Baskin et al., 2010 - Visualizing enveloping layer glycans during zebrafish early embryogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. S2

In vivo imaging of glycans during epiboly using microinjection of UDP-GalNAz and detection with copper-free click chemistry. Zebrafish embryos were microinjected with 25 pmol of UDP-GalNAz (top) or UDP-GalNAc (bottom), along with the tracer dye rhodaminedextran, and allowed to develop to 50% epiboly (5.5 hpf, 1st and 5th rows), 65% epiboly (7 hpf, 2nd and 6th rows), 80% epiboly (8.5 hpf, 3rd and 7th rows), or 95% epiboly (9.5 hpf, 4th and 8th rows). Embryos were then reacted with DIFO-488 (100 μM, 1 h) and imaged by confocal microscopy. Shown are maximum intensity z-projection fluorescence images and corresponding brightfield images. Embryos are orientated with the animal pole up, but randomly with respect to the dorsal-ventral axis. Faint azide-independent signal at 50% epiboly is due to nonspecific uptake of DIFO-488 by rapidly endocytosing dorsal forerunner cells. Green, DIFO-488; red, rhodamine-dextran. Scale bar: 200 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA