ZFIN Image: Baskin et al., 2010, Fig. S5

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Figure Caption

Fig. S5 In vivo imaging of glycans during epiboly using microinjection of GalNAz and detection with copper-free click chemistry. Zebrafish embryos were microinjected with 25 pmol of GalNAz (top) or no sugar (bottom), along with the tracer dye rhodamine-dextran, and allowed to develop to 50% epiboly (5.5 hpf, 1st and 4th rows), 65% epiboly (7 hpf, 2nd and 5th rows), or 95% epiboly (9.5 hpf, 3rd and 6th rows). Embryos were then reacted with DIFO-488 (100 μM, 1 h) and imaged by confocal microscopy. Shown are maximum intensity z-projection fluorescence images and corresponding brightfield images. Green, DIFO-488; red, rhodaminedextran. Scale bar: 200 μm.

Acknowledgments

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