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ZFIN ID: ZDB-FIG-100611-2
Riley et al., 2004 - Rhombomere boundaries are Wnt signaling centers that regulate metameric patterning in the zebrafish hindbrain. Developmental dynamics : an official publication of the American Association of Anatomists   231(2):278-291 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Prim-15

Fig. 2 Hindbrain development in wnt-deficient embryos. A-D: Expression of dlA at 30 hours postfertilization (hpf) in a Dfw5 mutant (A), a Dfw5 mutant injected with wnt3a-morpholino oligomers (MOs) (B), a wild-type embryo coinjected with wnt3a-MO and wnt8b-MO (C), and a Dfw5 mutant coinjected with wnt3a-MO and wnt8b-MO (D). E,F: Expression of erm at 30 hpf in a wild-type embryo (E) and a Dfw5 mutant coinjected with wnt3a-MO and wnt8b-MO (F). The otic vesicles (ov) and optic tectum (tec) are indicated to serve as landmarks. G,H: Expression of ephA4 at 24 hpf in a wild-type embryo (G) and a Dfw5 mutant coinjected with wnt3a-MO and wnt8b-MO (H). I,J: Expression of hoxb1a at 30 hpf in a wild-type embryo (I) and a Dfw5 mutant coinjected with wnt3a-MO and wnt8b-MO (J). K,L: Anti-acetylated tubulin staining of reticulospinal neurons (rsn) at 24 hpf in a wild-type embryo (K) and a Dfw5 mutant coinjected with wnt3a-MO and wnt8b-MO (L). M,N: Immunostaining with zn8 antibody at 30 hpf in a wild-type embryo (M) and a Dfw5 mutant coinjected with wnt3a-MO and wnt8b-MO (N). The zn8 antibody recognizes a cell adhesion molecule related to DM-Grasp (Fashena and Westerfield, [1999]) and normally labels commissural neurons (cn), some cranial ganglia, pharyngeal arches, floor plate, and heart (Trevarrow et al., [1990]). Commissural neurons are not produced in the wnt-deficient embryo. O,P: Immunostaining with a combination of four antibodies, zrf1-zrf4 (Trevarrow et al., [1990]), was used to label radial glia (rg) at 30 hpf in a wild-type (O) and a Dfw5 mutant coinjected with wnt3a-MO and wnt8b-MO (P). Images show dorsal views with anterior to the top (A-N) or lateral views with anterior to the left (O,P). Numbers mark locations of the corresponding rhombomeres. Scale bar in P = 60 μm in O,P, 70 μm in A-D,G-N, 85 μm in E,F.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dla AB + MO2-wnt3a + MO2-wnt8b standard conditions Prim-15 hindbrain ISH
Df(Chr23:acvr1ba,sp5l,wnt1,wnt10b)w5/w5 standard conditions Prim-15 hindbrain ISH
Df(Chr23:acvr1ba,sp5l,wnt1,wnt10b)w5/w5 + MO2-wnt3a standard conditions Prim-15 hindbrain ISH
Df(Chr23:acvr1ba,sp5l,wnt1,wnt10b)w5/w5 + MO2-wnt3a + MO2-wnt8b standard conditions Prim-15 hindbrain ISH
epha4a AB standard conditions Prim-5 rhombomere 3 ISH
Prim-5 rhombomere 5 ISH
Df(Chr23:acvr1ba,sp5l,wnt1,wnt10b)w5/w5 + MO2-wnt3a + MO2-wnt8b standard conditions Prim-5 rhombomere 3 ISH
Prim-5 rhombomere 5 ISH
etv5b AB standard conditions Prim-15 optic tectum ISH
Prim-15 otic vesicle ISH
Prim-15 rhombomere ISH
Df(Chr23:acvr1ba,sp5l,wnt1,wnt10b)w5/w5 + MO2-wnt3a + MO2-wnt8b standard conditions Prim-15 hindbrain ISH
Prim-15 optic tectum ISH
Prim-15 otic vesicle ISH
hoxb1a AB standard conditions Prim-5 rhombomere 4 ISH
Df(Chr23:acvr1ba,sp5l,wnt1,wnt10b)w5/w5 + MO2-wnt3a + MO2-wnt8b standard conditions Prim-5 rhombomere 4 ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-tuba IHC AB standard conditions Prim-5 rhombomere primary neuron
IHC Df(Chr23:acvr1ba,sp5l,wnt1,wnt10b)w5/w5 + MO2-wnt3a + MO2-wnt8b standard conditions Prim-5 rhombomere primary neuron
zn-8 IHC AB standard conditions Prim-15 hindbrain commissure neuron
IHC Df(Chr23:acvr1ba,sp5l,wnt1,wnt10b)w5/w5 + MO2-wnt3a + MO2-wnt8b standard conditions Prim-15 Not Detected hindbrain commissure neuron
zrf-1 IHC AB standard conditions Prim-15 radial glial cell
IHC Df(Chr23:acvr1ba,sp5l,wnt1,wnt10b)w5/w5 + MO2-wnt3a + MO2-wnt8b standard conditions Prim-15 radial glial cell
zrf-2 IHC AB standard conditions Prim-15 radial glial cell
IHC Df(Chr23:acvr1ba,sp5l,wnt1,wnt10b)w5/w5 + MO2-wnt3a + MO2-wnt8b standard conditions Prim-15 radial glial cell
zrf-3 IHC AB standard conditions Prim-15 radial glial cell
IHC Df(Chr23:acvr1ba,sp5l,wnt1,wnt10b)w5/w5 + MO2-wnt3a + MO2-wnt8b standard conditions Prim-15 radial glial cell
zrf-4 IHC AB standard conditions Prim-15 radial glial cell
IHC Df(Chr23:acvr1ba,sp5l,wnt1,wnt10b)w5/w5 + MO2-wnt3a + MO2-wnt8b standard conditions Prim-15 radial glial cell
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-wnt3a + MO2-wnt8b standard conditions Prim-15 embryonic pattern specification disrupted, abnormal
Prim-15 hindbrain disorganized, abnormal
Df(Chr23:acvr1ba,sp5l,wnt1,wnt10b)w5/w5 standard conditions Prim-15 hindbrain morphology, normal
Df(Chr23:acvr1ba,sp5l,wnt1,wnt10b)w5/w5 + MO2-wnt3a standard conditions Prim-15 embryonic pattern specification disrupted, abnormal
Prim-15 hindbrain morphology, abnormal
Df(Chr23:acvr1ba,sp5l,wnt1,wnt10b)w5/w5 + MO2-wnt3a + MO2-wnt8b standard conditions Prim-5 axon guidance disrupted, abnormal
Prim-5 rhombomere primary neuron crowded, abnormal
Prim-5 rhombomere primary neuron disorganized, abnormal
Prim-15 embryonic pattern specification disrupted, abnormal
Prim-15 hindbrain disorganized, abnormal
Prim-15 hindbrain commissure neuron absent, abnormal
Prim-15 radial glial cell cell projection irregular spatial pattern, abnormal
Prim-15 radial glial cell cell projection tangled, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.