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ZIRC
ZFIN ID: ZDB-FIG-100603-22
Deacon et al., 2010 - The miR-143-adducin3 pathway is essential for cardiac chamber morphogenesis. Development (Cambridge, England)   137(11):1887-1896 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 7 Add3 functions cell-autonomously to affect F-actin localization. (A-C) Flattened z-stacks showing phalloidin (red) and anti-DM-GRASP (green) labeling of 48 hpf WT, MO143 and MOadd3TP zebrafish cardiomyocytes within the OC. (D) The construct used to overexpress GFP and add3 (no 3′ UTR). (E,F) Cell circularity (E) and area (F) in mosaic hearts. Add3-overexpressing OC myocytes are statistically more circular (blue bar) than OC CMs labeled with GFP alone (green bar). *, P=0.00045. Error bars represent ± s.e.m. (G-N) Samples of the raw data used to generate the bar charts in E and F. Asterisks indicate GFP+ cells. (G-L) Head-on view of the ventricular OC. (M,N) Cross-section through the ventricle. (G) Merged confocal image showing WT GFP+ cells (green) and F-actin distribution (red). (H) Same image as in G but showing phalloidin staining alone. Abundant intracellular F-actin fibers can be seen in elongated GFP+ OC cells. (I,K,M) Merged confocal images showing add3-overexpressing GFP+ cells (green) and F-actin distribution (red). (J,L,N) The same images as in I,K,M but showing phalloidin staining alone. (I,J) Intracellular fibers are more visible in elongated WT cells than in add3-overexpressing neighbors (green). (K-N) Approximately half of the add3-overexpressing cells are extruded from the ventricular wall and show obvious cortical F-actin localization as compared with their WT (GFP-) neighbors.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
alcama zn-8 WT control Long-pec cardiac muscle cell plasma membrane IHC
zn-8 WT + MO1-add3a standard conditions Long-pec cardiac muscle cell plasma membrane IHC
zn-8 WT + MO1-mir143 standard conditions Long-pec cardiac muscle cell plasma membrane IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-add3a standard conditions Long-pec regulation of actin cytoskeleton reorganization decreased process quality, abnormal
Long-pec ventricular myocardium filamentous actin mislocalised, abnormal
WT + MO1-mir143 standard conditions Long-pec regulation of actin cytoskeleton reorganization decreased process quality, abnormal
Long-pec ventricular myocardium filamentous actin mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development