FIGURE

Fig. S1

ID
ZDB-FIG-100603-23
Publication
Deacon et al., 2010 - The miR-143-adducin3 pathway is essential for cardiac chamber morphogenesis
Other Figures
All Figure Page
Back to All Figure Page
Fig. S1

Injection of morpholinos that block Dicer cleavage of pre-miR-143 phenocopy MO143-injected embryos. (A-F) Embryos injected with two morpholinos that block Dicer cleavage on the guide (gDi143; A-C) or star (sDi143; D-F) strands of pre-miR-143 exhibit deflated, non-contractile ventricles and enlarged atria (C,F) that closely mimic hearts in MO143-injected embryos (as shown in Fig. 2F). (G) Diagram of pre-miR-143 and the target sites for three morpholinos used to inhibit Dicer and Drosha cleavage (MO143) or Dicer cleavage alone (gDi14; sDi143). (H) gDi143 and sDi143 interfere with miR-143 biosynthesis. Northern analysis of miR-143 in total RNA samples isolated from WT, gDi143 or sDi143 animals at ∼2.5 dpf. No mature miR-143 can be visualized in morphants, whereas the presence of an internal ribosomal RNA (rRNA) band appears unaltered.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development