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ZFIN ID: ZDB-FIG-100506-15
Nguyen et al., 2010 - The Paf1 complex component Leo1 is essential for cardiac and neural crest development in zebrafish. Developmental Biology   341(1):167-175 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec

Fig. 4 Cardiac differentiation phenotypes of leo1LA1186. Normally, vmhc expression is restricted in the ventricle in wild-type embryos (A), but is expanded into the atrium in leo1LA1186 mutants (B). Expression of amhc, however, is restricted properly to the atrial chamber in both wild-type (C) and leo1LA1186 mutant embryos (D). Notch1b transcripts are detected in the endocardium at the AV boundary of wild-type embryos (E), but are absent in leo1LA1186 mutants (F). Expression of bmp4 (G), versican (I), and wnt2bb (K) are detected in the myocardium of the AV boundary in wild-type embryos. Both bmp4 (H) and versican (J) transcripts are no longer restricted in leo1LA1186 mutants, while wnt2bb is not detected in mutant embryos (L). Dashed lines (in A–D) and arrows (in E, G, I, K) mark the boundary of atrium and ventricle.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bmp4 WT standard conditions Long-pec atrioventricular canal ISH
leo1la1186/la1186 standard conditions Long-pec heart ISH
myh6 WT standard conditions Long-pec atrium ISH
leo1la1186/la1186 standard conditions Long-pec atrium ISH
myh7 WT standard conditions Long-pec cardiac ventricle ISH
leo1la1186/la1186 standard conditions Long-pec atrium ISH
Long-pec cardiac ventricle ISH
notch1b WT standard conditions Long-pec atrioventricular canal ISH
leo1la1186/la1186 standard conditions Long-pec Not Detected atrioventricular canal ISH
vcana WT standard conditions Long-pec atrioventricular canal ISH
leo1la1186/la1186 standard conditions Long-pec heart ISH
wnt2bb WT standard conditions Long-pec atrioventricular canal ISH
leo1la1186/la1186 standard conditions Long-pec heart ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 341(1), Nguyen, C.T., Langenbacher, A., Hsieh, M., and Chen, J.N., The Paf1 complex component Leo1 is essential for cardiac and neural crest development in zebrafish, 167-175, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.