FIGURE

Fig. S5

ID

ZDB-FIG-100429-20

Publication

Gates et al., 2010 - The transcriptional repressor REST/NRSF modulates hedgehog signaling

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Fig. S5

rest morphant phenotypes are specific to Rest knockdown. Lateral views, 1dpf embryos stained with nkx2.2a (A-D) or pax 3 antisense riboprobes (E-H). Hindbrain, anterior trunk views of embryos treated with control MO (A, C) or translation blocking rest ATG MO (B, D) and co-injected with shh mRNA (C, D) rest ATG MO sensitizes embryos to ectopic Hh signaling, (compare D to C). This phenocopies the effects of the rest splice blocking MO. (E-H) Injection of shh mRNA represses pax3 expression (F, compare with E). The heightened effect of exogenous Shh in rest morphants (G, compare with F) is reversed by rest mRNA (H, compare with G).

Expression Data

Gene:	nkx2.2a
Fish:	WT WT + MO2-rest
Knockdown Reagent:	MO2-rest
Anatomical Term:	hindbrain neural tube ventral region
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 340(2), Gates, K.P., Mentzer, L., Karlstrom, R.O., and Sirotkin, H.I., The transcriptional repressor REST/NRSF modulates hedgehog signaling, 293-305, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.