FIGURE

Fig. 5

ID

ZDB-FIG-100429-12

Publication

Gates et al., 2010 - The transcriptional repressor REST/NRSF modulates hedgehog signaling

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Fig. 5

Rest acts downstream of Smo in the Hh pathway. Lateral views of 24 hpf embryos injected with control MO (A, E) rest MO (B, F), dominant-negative PKA mRNA (dnPKA, C, G), or both (D, H) and stained for nkx2.2a. Embryos were placed in control media (A–D) or media containing 50 μM cyclopamine (E–H). Injection of dnPKA mRNA expands nkx2.2a expression (C). This expansion is augmented by co-injection with rest MO (D). CyA treatment partially attenuates the effects of dnPKA mRNA injection on nkx2.2a expression (G). dnPKA mRNA/rest mo injected embryos (F) are more resistant to CyA treatment then dnPKA treated embryos.

Expression Data

Gene:	nkx2.2a
Fish:	WT WT + MO1-rest
Condition:	chemical treatment: Cyclopamine
Knockdown Reagent:	MO1-rest
Anatomical Terms:	hindbrain neural tube ventral region spinal cord neural tube ventral region
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 340(2), Gates, K.P., Mentzer, L., Karlstrom, R.O., and Sirotkin, H.I., The transcriptional repressor REST/NRSF modulates hedgehog signaling, 293-305, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.