ZFIN Image: Gates et al., 2010, Fig. S5

Image

Figure Caption

Fig. S5 rest morphant phenotypes are specific to Rest knockdown. Lateral views, 1dpf embryos stained with nkx2.2a (A-D) or pax 3 antisense riboprobes (E-H). Hindbrain, anterior trunk views of embryos treated with control MO (A, C) or translation blocking rest ATG MO (B, D) and co-injected with shh mRNA (C, D) rest ATG MO sensitizes embryos to ectopic Hh signaling, (compare D to C). This phenocopies the effects of the rest splice blocking MO. (E-H) Injection of shh mRNA represses pax3 expression (F, compare with E). The heightened effect of exogenous Shh in rest morphants (G, compare with F) is reversed by rest mRNA (H, compare with G).

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 340(2), Gates, K.P., Mentzer, L., Karlstrom, R.O., and Sirotkin, H.I., The transcriptional repressor REST/NRSF modulates hedgehog signaling, 293-305, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.