FIGURE

Fig. 8

ID

ZDB-FIG-100429-16

Publication

Gates et al., 2010 - The transcriptional repressor REST/NRSF modulates hedgehog signaling

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Fig. 8

Rest mediated repression of Hh target gene expression in gli mutants requires Gli2a. dtr/gli1 mutants, lateral views, head and hindbrain, stained for nkx2.2a by whole mount RNA in situ hybridization. Embryos were injected with control MO (A), rest MO (B), shh mRNA (C), rest mo/shh mRNA (D), gli2a MO (E) or rest MO/gli2a MO (F). In dtr/gli1 mutants, nkx2.2a expression is absent in control-injected embryos (A). However, hindbrain expression is partially restored in dtr/gli1 mutants treated with rest morpholino (arrows, B). dtr mutants are largely refractory to exogenous shh mRNA (C) dtr/gli1 mutants treated with rest morpholino have a qualitatively different response to shh mRNA treatment (D). Gli2a knockdown results in decreased midbrain nkx2.2a expression (E) The restoration of hindbrain nkx2.2a expression in dtr mutants produced by Rest knockdown (arrows, inset in B) is eliminated by simultaneous knockdown of Gli2a (compare F with B). This reveals that the enhancement of Hh signaling produced by Rest knockdown requires Gli2a and Gli1.

Expression Data

Gene:	nkx2.2a
Fish:	gli1^{te370a/te370a} gli1^{te370a/te370a} + MO1-gli2a gli1^{te370a/te370a} + MO1-rest gli1^{te370a/te370a} + MO1-gli2a + MO1-rest
Knockdown Reagents:	MO1-gli2a MO1-rest
Anatomical Term:	hindbrain neural tube
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 340(2), Gates, K.P., Mentzer, L., Karlstrom, R.O., and Sirotkin, H.I., The transcriptional repressor REST/NRSF modulates hedgehog signaling, 293-305, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.