ZFIN ID: ZDB-FIG-100309-74
Teoh et al., 2010 - Pbx1 is essential for growth of zebrafish swim bladder. Developmental dynamics : an official publication of the American Association of Anatomists   239(3):865-874 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-25 to Day 6
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-25 to Day 6

Fig. 5 Morpholino-mediated knockdown of pbx1 disrupts swim bladder, pancreas, and pharyngeal arches development. A,B: Swim bladder development in 6-dpf gut GFP transgenic embryo injected with StdMO (A) and Pbx1MO (B) showing non-inflated swim bladder in pbx1 morphants. C-H: In situ hybridization of swim bladder markers prdc, foxA3, and anxa5 using embryos injected with StdMO and Pbx1MO at 36 (C, D), 48 (E,F), and 60 hpf (G,H). I,J: In situ hybridization for anxa5 in 60-hpf embryos injected with Pbx1-Ex2MM (I) and Pbx1-Ex2MO (J). K,L: Expression of pbx1 in mutants deficient in hedgehog signaling. Reduced pbx1 expression in the syu mutant at 72 hpf (K) and absence of expression of pbx1 in the swim bladder of smu mutant at 72 hpf (L). M-P: Splice-blocking morpholino-mediated knockdown of pbx2 or pbx4 disrupts swim bladder development. In situ hybridization for anxa5 in 60-hpf embryos injected with Pbx2-Ex4MM (M) and Pbx2-Ex4MO (N), Pbx4-Ex3MM (O), and Pbx4-Ex3MO (P). Q,R: In situ hybridization for pancreas marker, pdx-1, in 36-hpf embryos injected with StdMO (Q) and Pbx1MO (R). S,T: In situ hybridization for dlx2a, a pharyngeal arches marker in 36-hpf embryos injected with StdMO (S) and Pbx1MO (T). Red arrow, swim bladder; black arrow, pancreas; blue arrow, posterior pharyngeal arches.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
anxa5b WT control Pec-fin swim bladder ISH
WT + MO1-pbx1a standard conditions Pec-fin Not Detected swim bladder ISH
WT + MO2-pbx1a standard conditions Pec-fin Not Detected swim bladder ISH
WT + MO4-pbx2 standard conditions Pec-fin swim bladder ISH
WT + MO5-pbx4 standard conditions Pec-fin Not Detected swim bladder ISH
dlx2a WT control Prim-25 pharyngeal arch ISH
WT + MO1-pbx1a standard conditions Prim-25 pharyngeal arch ISH
foxa3 WT control Pec-fin swim bladder ISH
WT + MO1-pbx1a standard conditions Pec-fin swim bladder ISH
GFP s854Tg control Day 6 swim bladder IFL
s854Tg + MO1-pbx1a standard conditions Day 6 swim bladder IFL
grem2b WT control Prim-25 swim bladder bud ISH
WT + MO1-pbx1a standard conditions Prim-25 swim bladder bud ISH
pbx1a shhaunspecified/unspecified standard conditions Pec-fin swim bladder ISH
smounspecified/unspecified standard conditions Pec-fin Not Detected swim bladder ISH
pdx1 WT control Prim-25 pancreatic bud ISH
WT + MO1-pbx1a standard conditions Prim-25 pancreatic bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-pbx1a standard conditions Prim-25 pancreas primordium physical object quality, abnormal
Prim-25 pharyngeal arch physical object quality, abnormal
Pec-fin swim bladder morphogenesis process quality, abnormal
Pec-fin swim bladder morphology, abnormal
WT + MO2-pbx1a standard conditions Pec-fin swim bladder physical object quality, abnormal
WT + MO5-pbx4 standard conditions Pec-fin swim bladder physical object quality, abnormal
s854Tg + MO1-pbx1a standard conditions Day 6 swim bladder inflation arrested, abnormal
Day 6 swim bladder morphogenesis process quality, abnormal
shhaunspecified/unspecified standard conditions Pec-fin swim bladder physical object quality, abnormal
smounspecified/unspecified standard conditions Pec-fin swim bladder physical object quality, abnormal
Acknowledgments:
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