PUBLICATION

Pbx1 is essential for growth of zebrafish swim bladder

Authors
Teoh, P.H., Shu-Chien, A.C., and Chan, W.K.
ID
ZDB-PUB-100202-11
Date
2010
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   239(3): 865-874 (Journal)
Registered Authors
Chan, Woon-Khiong
Keywords
pbx1, swim bladder, zebrafish
MeSH Terms
  • Models, Genetic
  • Animals
  • Time Factors
  • Protein Isoforms
  • Gene Expression Regulation, Developmental*
  • Zebrafish
  • Zebrafish Proteins/metabolism*
  • Central Nervous System/embryology
  • Oligonucleotides, Antisense/metabolism
  • In Situ Hybridization
  • Transcription Factors/metabolism*
  • Developmental Biology/methods
  • Air Sacs/embryology*
  • RNA, Messenger/metabolism
  • Branchial Region/embryology
(all 15)
PubMed
20108353 Full text @ Dev. Dyn.
Abstract
pbx1, a TALE (three-amino acid loop extension) homeodomain transcription factor, is involved in a diverse range of developmental processes. We examined the expression of pbx1 during zebrafish development by in situ hybridization. pbx1 transcripts could be detected in the central nervous system and pharyngeal arches from 24 hpf onwards. In the swim bladder anlage, pbx1 was detected as early as 28 hpf, making it the earliest known marker for this organ. Morpholino-mediated gene knockdown of pbx1 revealed that the swim bladder failed to inflate, with eventual lethality occurring by 8 dpf. The knockdown of pbx1 did not perturb the expression of prdc and foxA3, with both early swim bladder markers appearing normally at 36 and 48 hpf, respectively. However, the expression of anxa5 was completely abolished by pbx1 knockdown at 60 hpf suggesting that pbx1 may be required during the late stage of swim bladder development.
Genes / Markers
Figures
Figure Gallery (5 images)
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO1-pbx1astandard conditionsPrim-25
WT + MO1-pbx1astandard conditionsPrim-25
WT + MO1-pbx1astandard conditionsPec-fin
WT + MO1-pbx1astandard conditionsPec-fin
WT + MO2-pbx1astandard conditionsPec-fin
WT + MO5-pbx4standard conditionsPec-fin
s854Tg + MO1-pbx1astandard conditionsDay 6
s854Tg + MO1-pbx1astandard conditionsDay 6
shhaunspecified/unspecifiedstandard conditionsPec-fin
smounspecified/unspecifiedstandard conditionsPec-fin
1 - 10 of 10
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
s854TgTransgenic Insertion
    shha_unspecified
      		Unspecified
      smo_unspecified
        		Unspecified
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        pbx1aMO1-pbx1aMRPHLNO
        pbx1aMO2-pbx1aMRPHLNO
        pbx2MO3-pbx2MRPHLNO
        pbx2MO4-pbx2MRPHLNO
        pbx4MO4-pbx4MRPHLNO
        pbx4MO5-pbx4MRPHLNO
        1 - 6 of 6
        Show
        Fish
        Fish
        s854Tg
        s854Tg + MO1-pbx1a
        shhaunspecified/unspecified
        smounspecified/unspecified
        WT
        WT + MO1-pbx1a
        WT + MO2-pbx1a
        WT + MO4-pbx2
        WT + MO5-pbx4
        1 - 9 of 9
        Show
        Antibodies
        No data available
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        GFPEFGGFP
        1 - 1 of 1
        Show
        Mapping
        No data available
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        Citation
        Teoh, P.H., Shu-Chien, A.C., and Chan, W.K. (2010) Pbx1 is essential for growth of zebrafish swim bladder. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(3):865-874.