Spatio-temporal expression of pbx1 at the trunk region. A-D: Posterior pharyngeal arches (5th-7th arches) seen as early as 28 hpf located bilaterally anterior to the first somite. A: Expression of pbx1 in the swim bladder anlage at 28 hpf. B: Swim bladder anlage at 32 hpf. C: Swim bladder bud at 48 hpf. D: Elongated swim bladder sac at 72 hpf. E: Inflated swim bladder at 6 dpf. F: prdc expression in the swim bladder overlapped with pbx1 at 32 hpf. G: foxA3 expression at the swim bladder bud and other gut-derived organs at 48 hpf. H: Colocalization of pbx1 (blue) and anxa5 (red) in the swim bladder at 60 hpf. I, intestine; L, liver; PP, posterior pharynx; P, pancreas; black arrow, swim bladder; red arrow, posterior pharyngeal arches.
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