ZFIN ID: ZDB-FIG-090415-15
Thompson et al., 1998 - The cloche and spadetail genes differentially affect hematopoiesis and vasculogenesis. Developmental Biology   197:248-269 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5

Fig. 3 Comparison of the expression patterns of seven genes involved in hematopoiesis and vasculogenesis in wild-type embryos and embryos homozygous for one of two different mutations. Whole-mount in situ hybridization of 24-h-old wild-type (A–G), spadetail (sptb104) (H–N), and cloche (clom39) (O–U) embryos with lmo2 (A, H, O), gata2 (B, I, P), c-myb (C, J, Q), gata1 (D, K, R), fli1 (E, L, S), flk1 (F, M, T), and flt4 (G, N). Arrowhead in A indicates trunk lmo2 expression. In spt mutant embryos, cells expressing lmo2 (H) and gata2 (I) are found in the morphologically distorted ICM region and tails. However, very few if any hematopoietic cells expressing gata1 (K) or c-myb (J) are seen in these embryos. The expression levels of the vascular markers fli1 (L), flk1 (M), and flt4 (N) appear normal in these embryos; however, the patterns are distorted by the abnormal somites. In clo mutant embryos, ICM cells and tail cells that express lmo2 (O) or gata2 (P) are not found. While the expression of fli1 (S), gata1 (R), c-myb (Q), flk1 (T), and flt4 (U) is missing in the anterior ICM of clo mutant embryos, a small number of posterior ICM and tail cells continue to express these markers (arrows in O–U). The expression of c-myb in the eye, gut, and skin-associated cells appears normal in all of these mutants (C, J, Q).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
fli1 WT standard conditions Prim-5 blood vasculature ISH
Prim-5 blood vessel ISH
npas4lDf(Chr13:lycat)m39(INDO) standard conditions Prim-5 blood vasculature ISH
Prim-5 blood vessel ISH
tbx16b104/b104(AB) standard conditions Prim-5 blood vasculature ISH
Prim-5 blood vessel ISH
flt4 WT standard conditions Prim-5 blood vasculature ISH
Prim-5 blood vessel ISH
npas4lDf(Chr13:lycat)m39(INDO) standard conditions Prim-5 blood vasculature ISH
Prim-5 blood vessel ISH
tbx16b104/b104(AB) standard conditions Prim-5 blood vasculature ISH
Prim-5 blood vessel ISH
gata1a WT standard conditions Prim-5 intermediate cell mass of mesoderm ISH
npas4lDf(Chr13:lycat)m39(INDO) standard conditions Prim-5 intermediate cell mass of mesoderm ISH
tbx16b104/b104(AB) standard conditions Prim-5 intermediate cell mass of mesoderm ISH
gata2a WT standard conditions Prim-5 intermediate cell mass of mesoderm ISH
npas4lDf(Chr13:lycat)m39(INDO) standard conditions Prim-5 Not Detected intermediate cell mass of mesoderm ISH
tbx16b104/b104(AB) standard conditions Prim-5 intermediate cell mass of mesoderm ISH
kdrl WT standard conditions Prim-5 blood vasculature ISH
Prim-5 blood vessel ISH
npas4lDf(Chr13:lycat)m39(INDO) standard conditions Prim-5 blood vasculature ISH
Prim-5 blood vessel ISH
tbx16b104/b104(AB) standard conditions Prim-5 blood vasculature ISH
Prim-5 blood vessel ISH
lmo2 WT standard conditions Prim-5 intermediate cell mass of mesoderm ISH
npas4lDf(Chr13:lycat)m39(INDO) standard conditions Prim-5 Not Detected intermediate cell mass of mesoderm ISH
tbx16b104/b104(AB) standard conditions Prim-5 intermediate cell mass of mesoderm ISH
myb WT standard conditions Prim-5 eye ISH
Prim-5 gut ISH
Prim-5 integument ISH
Prim-5 intermediate cell mass of mesoderm ISH
npas4lDf(Chr13:lycat)m39(INDO) standard conditions Prim-5 eye ISH
Prim-5 gut ISH
Prim-5 integument ISH
tbx16b104/b104(AB) standard conditions Prim-5 eye ISH
Prim-5 gut ISH
Prim-5 integument ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 197, Thompson, M.A., Ransom, D.G., Pratt, S.J., MacLennan, H., Kieran, M.W., Detrich,III, H.W., Vail, B., Huber, T.L., Paw, B., Brownlie, A.J., Oates, A.C., Fritz, A., Gates, MA., Amores, A., Bahary, N., Talbot, W.S., Her, H., Beier, D.R., Postlethwait, J.H., and Zon, L.I., The cloche and spadetail genes differentially affect hematopoiesis and vasculogenesis, 248-269, Copyright (1998) with permission from Elsevier. Full text @ Dev. Biol.