ZFIN ID: ZDB-FIG-090415-13
Thompson et al., 1998 - The cloche and spadetail genes differentially affect hematopoiesis and vasculogenesis. Developmental Biology   197:248-269 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 14-19 somites

Fig. 5 Deletion of the c-myb gene in b316 mutants does not result in a loss of gata1 expression in zebrafish embryonic erythroid cells. Whole-mount in situ hybridization of wild-type (A) and b316 (B) embryos at approximately 18 h postfertilization with gata1. Note that the b316 embryo development is arrested at about 5-somites, but that it appears to have a normal level of gata1 expression. PCR of DNA from wild-type or b316 embryos with two different c-myb primer pairs, and with primers specific for gata2, lmo2, and gata1 (C). c-myb is not amplified with either primer pair from DNA made from b316 embryos, while it is amplified from DNA made from wild-type embryos. gata2, lmo2, and gata1 all amplify in both b316 and wild-type embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gata1a Df(Chr23:myb)b316/b316 standard conditions 14-19 somites erythroid progenitor cell ISH
14-19 somites whole organism RTPCR
WT standard conditions 14-19 somites erythroid progenitor cell ISH
14-19 somites whole organism RTPCR
gata2a Df(Chr23:myb)b316/b316 standard conditions 14-19 somites whole organism RTPCR
WT standard conditions 14-19 somites whole organism RTPCR
lmo2 Df(Chr23:myb)b316/b316 standard conditions 14-19 somites whole organism RTPCR
WT standard conditions 14-19 somites whole organism RTPCR
myb Df(Chr23:myb)b316/b316 standard conditions 14-19 somites Not Detected whole organism RTPCR
WT standard conditions 14-19 somites whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 197, Thompson, M.A., Ransom, D.G., Pratt, S.J., MacLennan, H., Kieran, M.W., Detrich,III, H.W., Vail, B., Huber, T.L., Paw, B., Brownlie, A.J., Oates, A.C., Fritz, A., Gates, MA., Amores, A., Bahary, N., Talbot, W.S., Her, H., Beier, D.R., Postlethwait, J.H., and Zon, L.I., The cloche and spadetail genes differentially affect hematopoiesis and vasculogenesis, 248-269, Copyright (1998) with permission from Elsevier. Full text @ Dev. Biol.