PUBLICATION

The cloche and spadetail genes differentially affect hematopoiesis and vasculogenesis

Authors
Thompson, M.A., Ransom, D.G., Pratt, S.J., MacLennan, H., Kieran, M.W., Detrich,III, H.W., Vail, B., Huber, T.L., Paw, B., Brownlie, A.J., Oates, A.C., Fritz, A., Gates, MA., Amores, A., Bahary, N., Talbot, W.S., Her, H., Beier, D.R., Postlethwait, J.H., and Zon, L.I.
ID
ZDB-PUB-980701-14
Date
1998
Source
Developmental Biology   197: 248-269 (Journal)
Registered Authors
Amores, Angel, Bahary, Nathan, Beier, David R., Brownlie, Alison J., Detrich, H. William, Fritz, Andreas, Gates, Michael A., Huber, Tara, Kieran, Mark, Oates, Andrew, Paw, Barry, Postlethwait, John H., Pratt, Stephen J., Ransom, David G., Talbot, William S., Vail, Brenda, Zon, Leonard I.
Keywords
zebrafish; hematopoiesis; blood; Danio rerio
MeSH Terms
  • Gene Expression Regulation, Developmental
  • Base Sequence
  • Metalloproteins/genetics
  • Adaptor Proteins, Signal Transducing
  • Sequence Homology, Amino Acid
  • Animals
  • In Situ Hybridization
  • Transcription Factors
  • Polymerase Chain Reaction
  • Trans-Activators/genetics
  • DNA-Binding Proteins/genetics
  • Amino Acid Sequence
  • Proto-Oncogene Proteins/genetics
  • DNA Primers/genetics
  • Molecular Sequence Data
  • Humans
  • LIM Domain Proteins
  • Zebrafish Proteins
  • Mutation
  • Erythropoiesis/genetics
  • Hematopoiesis/genetics*
  • Blood Vessels/embryology*
  • Evolution, Molecular
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Proto-Oncogene Proteins c-myb
(all 26)
PubMed
9630750 Full text @ Dev. Biol.
Abstract
In vertebrates, hematopoietic and vascular progenitors develop from ventral mesoderm. The first primitive wave of hematopoiesis yields embryonic red blood cells, whereas progenitor cells of subsequent definitive waves form all hematopoietic cell lineages. In this report we examine the development of hematopoietic and vasculogenic cells in normal zebrafish and characterize defects in cloche and spadetail mutant embryos. The zebrafish homologs of lmo2, c-myb, fli1, flk1, and flt4 have been cloned and characterized in this study. Expression of these genes identifies embryonic regions that contain hematopoietic and vascular progenitor cells. The expression of c-myb also identifies definitive hematopoietic cells in the ventral wall of the dorsal aorta. Analysis of b316 mutant embryos that carry a deletion of the c-myb gene demonstrates that c-myb is not required for primitive erythropoiesis in zebrafish even though it is expressed in these cells. Both cloche and spadetail mutant embryos have defects in primitive hematopoiesis and definitive hematopoiesis. The cloche mutants also have significant decreases in vascular gene expression, whereas spadetail mutants expressed normal levels of these genes. These studies demonstrate that the molecular mechanisms that regulate hematopoiesis and vasculogenesis have been conserved throughout vertebrate evolution and the clo and spt genes are key regulators of these programs.
Genes / Markers
Figures
Figure Gallery (5 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b104
    Indel
    b316
      Deficiency
      m39
        Deficiency
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        Antibodies
        No data available
        Orthology
        Gene Orthology
        fli1
        lmo2
        myb
        1 - 3 of 3
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        Engineered Foreign Genes
        No data available
        Mapping
        No data available