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ZFIN ID: ZDB-FIG-090224-5
Meani et al., 2009 - The tumor suppressor PRDM5 regulates Wnt signaling at early stages of zebrafish development. PLoS One   4(1):e4273 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 90%-epiboly
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 90%-epiboly

Fig. 4 Analysis of CE movements at gastrulation.

A, E = diagrams illustrating the staining pattern. B–D: Dorsal views of zebrafish embryos at 90% epiboly stage, stained for hgg1 (h = rostral mesendoderm), ntl (n = notochord), dlx3 (b = neural border). White dashed lines mark the dlx3 signal, and correspond to the border between neural and non-neural ectoderm. F-H: dorsal views of zebrafish embryos at 90% epiboly stage, stained for rx3 (e+t = eye field+telencephalon), pax2a (mhb = midhindbrain boundary) and ntl (n = notochord). The embryos were uninjected (B, F), injected with 6 ng SBmo (C, G) or 150 pg hPRDM5 mRNA (D, H). White lines mark the distance between eye field and mid-hindbrain boundary.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctslb TU standard conditions 90%-epiboly mesoderm ISH
TU + MO2-prdm5 standard conditions 90%-epiboly mesoderm ISH
dlx3b TU standard conditions 90%-epiboly preplacodal ectoderm ISH
TU + MO2-prdm5 standard conditions 90%-epiboly preplacodal ectoderm ISH
pax2a TU standard conditions 90%-epiboly midbrain hindbrain boundary neural plate ISH
TU + MO2-prdm5 standard conditions 90%-epiboly midbrain hindbrain boundary neural plate ISH
rx3 TU standard conditions 90%-epiboly anterior neural plate ISH
TU + MO1-prdm5 + MO2-prdm5 standard conditions 90%-epiboly anterior neural plate ISH
tbxta TU standard conditions 90%-epiboly axial chorda mesoderm ISH
TU + MO2-prdm5 standard conditions 90%-epiboly axial chorda mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO2-prdm5 standard conditions 90%-epiboly anterior neural plate decreased length, abnormal
90%-epiboly mesoderm cellular motility, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One