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Fig. 7 A repressor form of Tcf3 can block neurogenesis upstream of sox4a. (A,B) Heat shock-induced expression of ΔTcf at 18 hpf eliminates expression of sox4a, both alone and in the presence of tcf3 morpholinos. (C,D) ΔTcf expression at 18 hpf completely blocks BrdU uptake at 24 hpf. (E,F) Expression of ΔTcf (green, visualized by GFP fusion) blocks expression of HuC/D (red), both alone and in the presence of tcf3 morpholinos. (G) Simultaneous overexpression of sox4a mRNA and the ΔTcf transgene leads to ectopic Hu-positive cells in the medial spinal cord (arrow). (H) Co-injection of tcf3 and sox4a morpholinos does not lead to ectopic Hu-positive cells in the medial spinal cord.