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ZFIN ID: ZDB-FIG-090220-61
Carreira-Barbosa et al., 2009 - Flamingo regulates epiboly and convergence/extension movements through cell cohesive and signalling functions during zebrafish gastrulation. Development (Cambridge, England)   136(3):383-392 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Bud
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Bud

Fig. 1 Injection of celsr1a and celsr1b morpholinos in MZord embryos leads to a defective epiboly phenotype during zebrafish gastrulation. MZord embryos (A,B,C,G,H) or MZord embryos injected with 0.4 pmoles each of celsr1a and celsr1b morpholinos (D,E,F,I,J) were visualised at tail-bud stage with markers, as indicated in the bottom right-hand corner, by in situ hybridisation. hgg1 was used to indicate the prechordal plate (pcp), ntl for the prospective notochord (n) and germ ring blastopore margin, and dlx3 for the anterior edge of the neural plate (A-F). Embryos were also visualised at 90% epiboly with phalloidin for actin (G,I) or anti-α-catenin antibody (H,J). An arrowhead represents the leading edge of the EVL with the actin cable being formed (I). An arrow indicates the leading edge of deep cells (J). Note that the deep cells are delayed from the leading edge of the enveloping layer in the mutant/morphant embryos. y, yolk. (K) Cell elongation of the EVL along the animal-vegetal (AV) axis and the mediolateral (ML) axis at 90% epiboly is quantified as AV/ML ratio (see the inset of G), and the measurement is made using ImageJ from 80 cells (four embryos of each group). Cells that are attached with the actin cable are expressed as `Row1', whereas cells that are not attached to that as `Row2/3'. Means and standard deviations (s.d.) are shown, and an asterisk indicates a statistically significant difference (P<0.05; Student's t-test).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctslb celsr2rw71/rw71; rw0Tg standard conditions Bud prechordal plate ISH
celsr2rw71/rw71; rw0Tg + MO3-celsr1b + MO4-celsr1a standard conditions Bud prechordal plate ISH
dlx3b celsr2rw71/rw71; rw0Tg standard conditions Bud anterior neural plate ISH
celsr2rw71/rw71; rw0Tg + MO3-celsr1b + MO4-celsr1a standard conditions Bud anterior neural plate ISH
tbxta celsr2rw71/rw71; rw0Tg standard conditions Bud margin ISH
Bud notochord ISH
celsr2rw71/rw71; rw0Tg + MO3-celsr1b + MO4-celsr1a standard conditions Bud margin ISH
Bud notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
celsr2rw71/rw71; rw0Tg + MO3-celsr1b + MO4-celsr1a standard conditions Bud epiboly involved in gastrulation with mouth forming second delayed, abnormal
Bud germ ring closure incomplete, abnormal
Bud notochord decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development