ZFIN ID: ZDB-FIG-080715-43
Martin et al., 2008 - Regulation of canonical Wnt signaling by Brachyury is essential for posterior mesoderm formation. Developmental Cell   15(1):121-133 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 75%-epiboly to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 2 Bra Knockdown Enhances the ntl Phenotype

(A) A schematic of Bra knockdown strategies. MO1 targets the bra splice acceptor site at the intron 1-exon 2 junction. Primers in the first and third exons were used to analyze the bra transcript in uninjected (UC) and MO1-injected embryos. The improperly spliced bra transcript results in a truncated protein that lacks the T box. MO2 targets the translation start site of bra.

(B–E) Expression of myoD at 24 hpf in an (B) uninjected +/+ or +/- sibling, (C) uninjected ntl mutant, (D) MO1-injected ntl mutant, and (E) MO2-injected ntl mutant.

(F–I) Expression of tbx6 in four-somite embryos.

(J–Q) Expression of ntl at 75% epiboly and bud. Arrows and arrowheads show diminished notochord expression.

Embryos are shown from a (B-E) lateral view with anterior to the left, (F-I) posterior view with dorsal to the top, (J-M) dorsal view with animal pole to the top, and (N-Q) dorsal view with anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myod1 WT standard conditions Prim-5 somite ISH
tbxtab195/b195 standard conditions Prim-5 somite ISH
tbxtab195/b195 + MO1-tbxtb + MO4-tp53 standard conditions Prim-5 somite ISH
tbxtab195/b195 + MO2-tbxtb standard conditions Prim-5 somite ISH
tbx16l WT standard conditions 1-4 somites paraxial mesoderm ISH
WT + MO2-tbxtb standard conditions 1-4 somites paraxial mesoderm ISH
tbxtab195/b195 standard conditions 1-4 somites paraxial mesoderm ISH
tbxtab195/b195 + MO2-tbxtb standard conditions 1-4 somites paraxial mesoderm ISH
tbxta WT standard conditions 75%-epiboly margin ISH
Bud margin ISH
Bud notochord ISH
WT + MO2-tbxtb standard conditions 75%-epiboly margin ISH
Bud margin ISH
Bud notochord ISH
tbxtab195/b195 standard conditions 75%-epiboly margin ISH
Bud margin ISH
Bud notochord ISH
tbxtab195/b195 + MO2-tbxtb standard conditions 75%-epiboly margin ISH
Bud margin ISH
Bud notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tbxtab195/b195 standard conditions Prim-5 post-vent region decreased length, abnormal
Prim-5 somite decreased amount, abnormal
Prim-5 somite decreased size, abnormal
Prim-5 somite morphology, abnormal
tbxtab195/b195 + MO1-tbxtb + MO4-tp53 standard conditions Prim-5 post-vent region decreased length, abnormal
Prim-5 somite decreased amount, abnormal
Prim-5 somite decreased size, abnormal
Prim-5 somite morphology, abnormal
tbxtab195/b195 + MO2-tbxtb standard conditions Prim-5 post-vent region decreased length, abnormal
Prim-5 somite decreased amount, abnormal
Prim-5 somite decreased size, abnormal
Prim-5 somite morphology, abnormal
Acknowledgments:
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Reprinted from Developmental Cell, 15(1), Martin, B.L., and Kimelman, D., Regulation of canonical Wnt signaling by Brachyury is essential for posterior mesoderm formation, 121-133, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell