Fig. 2
Bra Knockdown Enhances the ntl Phenotype (A) A schematic of Bra knockdown strategies. MO1 targets the bra splice acceptor site at the intron 1-exon 2 junction. Primers in the first and third exons were used to analyze the bra transcript in uninjected (UC) and MO1-injected embryos. The improperly spliced bra transcript results in a truncated protein that lacks the T box. MO2 targets the translation start site of bra. (B–E) Expression of myoD at 24 hpf in an (B) uninjected +/+ or +/- sibling, (C) uninjected ntl mutant, (D) MO1-injected ntl mutant, and (E) MO2-injected ntl mutant. (F–I) Expression of tbx6 in four-somite embryos. (J–Q) Expression of ntl at 75% epiboly and bud. Arrows and arrowheads show diminished notochord expression. Embryos are shown from a (B-E) lateral view with anterior to the left, (F-I) posterior view with dorsal to the top, (J-M) dorsal view with animal pole to the top, and (N-Q) dorsal view with anterior to the left. |
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| Fish: | |
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| Anatomical Terms: | |
| Stage Range: | 75%-epiboly to Prim-5 |
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| Knockdown Reagents: | |
| Observed In: | |
| Stage: | Prim-5 |
Reprinted from Developmental Cell, 15(1), Martin, B.L., and Kimelman, D., Regulation of canonical Wnt signaling by Brachyury is essential for posterior mesoderm formation, 121-133, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell