FIGURE

Fig. 4

ID

ZDB-FIG-080505-49

Publication

Hawkins et al., 2008 - The small molecule Mek1/2 inhibitor U0126 disrupts the chordamesoderm to notochord transition in zebrafish

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Fig. 4

Genes important in notochord differentiation exhibit two types of regulatory pattern during chordamesoderm to mature NC transition. A-H: Expression of ntl is normal during early NC development (A-D) in treated embryos but fails to get downregulated during the chordamesoderm to mature NC transition step (E-H). (A, B: 7 hpf; C, D: 12 hpf; E-H 24 hpf.) This pattern of developmental arrest occurs in ehh (I, J), shh (L, K), col2α1 (M, N) and hsp47 (O, P) expression. Q-X: mRNA levels of fkd1 (Q, R), fkd4 (S, T), spondin2 (U, V) and mindin (W, X) however decreases normally upon chordamesoderm to mature NC transition. Notice the unchanged expression of marker genes in the floor plate (M-X) and in the hypochord (O, P, U-X) of U0126-treated embryos. Panels: A, C, E, G, I, K, M, O, Q, S, U, W: wild type. Panels: B, D, F, H, J, L, N, P, R, T, V, X: U0126-treated embryos.

Expression Data

Genes:	col2a1a foxa foxa2 ihhb serpinh1b shha spon1b spon2a tbxta
Fish:	WT
Condition:	chemical treatment: (2Z,3Z)-bis{amino[(2-aminophenyl)sulfanyl]methylidene}butanedinitrile
Anatomical Terms:	floor plate hypochord margin notochord shield tail bud
Stage Range:	Shield to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment: (2Z,3Z)-bis{amino[(2-aminophenyl)sulfanyl]methylidene}butanedinitrile
Observed In:	notochord
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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