ZFIN Image: Hawkins et al., 2008, Fig. 4

Image

Figure Caption

Fig. 4 Genes important in notochord differentiation exhibit two types of regulatory pattern during chordamesoderm to mature NC transition. A-H: Expression of ntl is normal during early NC development (A-D) in treated embryos but fails to get downregulated during the chordamesoderm to mature NC transition step (E-H). (A, B: 7 hpf; C, D: 12 hpf; E-H 24 hpf.) This pattern of developmental arrest occurs in ehh (I, J), shh (L, K), col2α1 (M, N) and hsp47 (O, P) expression. Q-X: mRNA levels of fkd1 (Q, R), fkd4 (S, T), spondin2 (U, V) and mindin (W, X) however decreases normally upon chordamesoderm to mature NC transition. Notice the unchanged expression of marker genes in the floor plate (M-X) and in the hypochord (O, P, U-X) of U0126-treated embryos. Panels: A, C, E, G, I, K, M, O, Q, S, U, W: wild type. Panels: B, D, F, H, J, L, N, P, R, T, V, X: U0126-treated embryos.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.