FIGURE

Fig. 5

ID

ZDB-FIG-080505-50

Publication

Hawkins et al., 2008 - The small molecule Mek1/2 inhibitor U0126 disrupts the chordamesoderm to notochord transition in zebrafish

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Fig. 5

Not all tissues receiving notochord-emitted signals are affected by U0126 treatment. A-D: flk-1 expression reveals defects in dorsal aorta differentiation (green arrow in panel D) and in the sprouting of intersomitic vessels. Notice that both lack of sprouting (yellow arrows) and double sprouting (red arrow) occurs in U0126-treated embryos (D). Expression of the early muscle differentiation marker myoD is normal in treated embryos (E, F) but the muscle pioneer cell marker eng2 is upregulated (G, H). The floor plate of the neural tube differentiates normally (I, J and also Fig. 4/M–X). Isl-1 expression shows that the sensory Rohon-Beard neurons and motoneurons both develop properly in treated embryos (K, L)

Expression Data

Genes:	en2a isl1a kdrl myod1 shhb
Fish:	WT
Condition:	chemical treatment: (2Z,3Z)-bis{amino[(2-aminophenyl)sulfanyl]methylidene}butanedinitrile
Anatomical Terms:	dorsal aorta floor plate intersegmental vessel motor neuron muscle pioneer myotome Rohon-Beard neuron
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment: (2Z,3Z)-bis{amino[(2-aminophenyl)sulfanyl]methylidene}butanedinitrile
Observed In:	trunk vasculature
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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