FIGURE

Fig. 6

ID

ZDB-FIG-080401-6

Publication

Nakayama et al., 2008 - Fgf19 is required for zebrafish lens and retina development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Nasal–temporal patterning of the retina in Fgf19 MO1-injected and Fgf19 mRNA-injected embryos. (a, d, f, h, k, n) Control embryos at 28 hpf. (b, e, g, i, l, o) Fgf19 MO1-injected embryos at 28 hpf. (c, j, m) Fgf19 mRNA-injected embryos at 28 hpf. (a-c) The expression of ephrin A3 was completely lost in the nasal half of the prospective retina of Fgf19 MO1-injected embryos, while the expression of ephrin A3 partially expanded into the temporal retina in Fgf19 mRNA-injected embryos. (d-g) The expression of ephrin A5a and foxg1 was severely reduced in the nasal retina of Fgf19 MO1-injected embryos. (h-j) The expression of eph A4b in the temporal half of the prospective retina partially expanded into the nasal retina in Fgf19 MO1-injected embryos, while the expression of eph A4b was decreased in the temporal retina of Fgf19 mRNA-injected embryos. (k, l) The nasal expansion of tbx5 expressetina in Fgf19 MO1-injected embryos, while the expression of eph A4b was decreased in the temporal retina of tbx5 was not affected in the dorsal–ventral extent. (m) The expression of tbx5 was severely decreased in Fgf19 mRNA-injected embryos. (n, o) A partial temporal shift of vax2 expression in the ventral retina was observed in Fgf19 MO1-injected embryos. (p, q) An increased expression of pax2.1 in the optic stalk was detected in Fgf19 MO1-injected embryos at 18 hpf. (r) The expression of pax2.1 was unaffected in Fgf19 mRNA-injected embryos. (s, t) A decreased expression of pax6a in neural retina was detected in the ventral retina of Fgf19 MO1-injected embryos at 20 hpf. (a–o) Lateral views with anterior to the left and dorsal to the top. (p–t) Dorsal views with anterior to the top.

Expression Data

Genes:	efna3b efna5a epha4b foxg1a pax2a pax6a tbx5a vax2
Fish:	WT WT + MO1-fgf19
Knockdown Reagent:	MO1-fgf19
Anatomical Terms:	optic stalk optic vesicle presumptive neural retina
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 313(2), Nakayama, Y., Miyake, A., Nakagawa, Y., Mido, T., Yoshikawa, M., Konishi, M., and Itoh, N., Fgf19 is required for zebrafish lens and retina development, 752-766, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.