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ZFIN ID: ZDB-FIG-080326-22
Smith et al., 2008 - Rotation and asymmetric development of the zebrafish heart requires directed migration of cardiac progenitor cells. Developmental Cell   14(2):287-297 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 20-25 somites to Prim-15

Fig. 3 Has2 Expression in CPCs Is Required for Jogging and Rotation of the Cardiac Cone (A–L) One- and two-color ISH (probes indicated at bottom) on wild-type embryos (A–G, I, and K) or embryos injected with a southpaw MO (J) or a has2 MO (H and L) during various stages (indicated at the top) of heart tube formation. Evident is the dynamic expression pattern of has2 in the CPCs. All images are dorsal views with anterior to the top and left to the left. (M and N) Combined ISH with an antisense lefty2 riboprobe (blue) and anti-tropomyosin antibody staining (brown, labeling all cardiomyocytes) of a control MO-injected embryo (M) or has2 MO-injected embryo (N). (O) Selected images of a confocal time-lapse recording of a tg(cmlc2:gfp) embryo injected with a has2 MO starting at the 23-somite stage. Individual GFP-positive cells were tracked and color coded according to their location within the cardiac field at the 23-somite stage. Dorsal views with anterior to the top. (P) Displacement rates of CPCs localized at different positions within the cardiac field (color corresponds to position indicated in the confocal image) calculated from time-lapse recording shown in (O) (n = 55, mean ± SEM). Evident is the absence of leftward displacement of the CPCs and their equal displacement rates. (Q) Selected images of a confocal time-lapse recording shown in (O) with individual cells marked (red, light blue, dark blue, and green for atrial cells located lateral and pink for ventricular cells located medial) and tracked over a 250 min period. (R) Quantification of clockwise cardiac cone rotation in wild-type and has2 MO-injected embryos (mean ± SEM).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP twu34Tg + MO1-has2 + MO2-has2 standard conditions 20-25 somites to Prim-5 cardiac muscle cell IFL
20-25 somites to Prim-5 heart rudiment IFL
has2 WT standard conditions 14-19 somites heart primordium ISH
20-25 somites heart rudiment ISH
26+ somites heart rudiment ISH
WT + MO1-spaw + MO2-spaw standard conditions 20-25 somites heart rudiment ISH
lft2 WT control Prim-15 heart tube ISH
WT + MO1-has2 + MO2-has2 standard conditions Prim-15 heart tube ISH
myh6 WT standard conditions 20-25 somites heart rudiment ISH
myh7 WT standard conditions 20-25 somites heart rudiment ISH
myl7 WT standard conditions 14-19 somites heart primordium ISH
20-25 somites heart rudiment ISH
26+ somites heart rudiment ISH
Prim-15 heart tube ISH
WT + MO1-has2 + MO2-has2 standard conditions 20-25 somites heart rudiment ISH
Prim-15 heart tube ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-has2 + MO2-has2 standard conditions Prim-15 determination of left/right symmetry disrupted, abnormal
Prim-15 heart looping disrupted, abnormal
WT + MO1-spaw + MO2-spaw standard conditions 20-25 somites determination of left/right symmetry disrupted, abnormal
20-25 somites heart looping disrupted, abnormal
twu34Tg + MO1-has2 + MO2-has2 standard conditions 20-25 somites - Prim-5 determination of left/right symmetry disrupted, abnormal
20-25 somites - Prim-5 heart looping disrupted, abnormal
20-25 somites - Prim-5 heart rudiment rotated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 14(2), Smith, K.A., Chocron, S., von der Hardt, S., de Pater, E., Soufan, A., Bussmann, J., Schulte-Merker, S., Hammerschmidt, M., and Bakkers, J., Rotation and asymmetric development of the zebrafish heart requires directed migration of cardiac progenitor cells, 287-297, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell