PUBLICATION

Rotation and asymmetric development of the zebrafish heart requires directed migration of cardiac progenitor cells

Authors
Smith, K.A., Chocron, S., von der Hardt, S., de Pater, E., Soufan, A., Bussmann, J., Schulte-Merker, S., Hammerschmidt, M., and Bakkers, J.
ID
ZDB-PUB-080309-9
Date
2008
Source
Developmental Cell   14(2): 287-297 (Journal)
Registered Authors
Bakkers, Jeroen, Bussmann, Jeroen, Chocron, Sonja, Hammerschmidt, Matthias, Schulte-Merker, Stefan, Smith, Kelly, von der Hardt, Sophia
Keywords
none
MeSH Terms
  • Animals
  • Body Patterning*/drug effects
  • Bone Morphogenetic Proteins/pharmacology
  • Cell Lineage/drug effects
  • Cell Movement*/drug effects
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/drug effects
  • Glucuronosyltransferase/metabolism
  • Heart/drug effects
  • Heart/embryology*
  • Humans
  • Left-Right Determination Factors
  • Mesoderm/cytology
  • Mesoderm/drug effects
  • Mutation/genetics
  • Myocardium/cytology*
  • Rotation*
  • Signal Transduction/drug effects
  • Smad Proteins/metabolism
  • Stem Cells/cytology*
  • Stem Cells/drug effects
  • Transforming Growth Factor beta/metabolism
  • Zebrafish/embryology*
  • Zebrafish Proteins/metabolism
PubMed
18267096 Full text @ Dev. Cell
Abstract
We have used high-resolution 4D imaging of cardiac progenitor cells (CPCs) in zebrafish to investigate the earliest left-right asymmetric movements during cardiac morphogenesis. Differential migratory behavior within the heart field was observed, resulting in a rotation of the heart tube. The leftward displacement and rotation of the tube requires hyaluronan synthase 2 expression within the CPCs. Furthermore, by reducing or ectopically activating BMP signaling or by implantation of BMP beads we could demonstrate that BMP signaling, which is asymmetrically activated in the lateral plate mesoderm and regulated by early left-right signals, is required to direct CPC migration and cardiac rotation. Together, these results support a model in which CPCs migrate toward a BMP source during development of the linear heart tube, providing a mechanism by which the left-right axis drives asymmetric development of the vertebrate heart.
Genes / Markers
Figures
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Expression
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Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes