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ZFIN ID: ZDB-FIG-080326-24
Smith et al., 2008 - Rotation and asymmetric development of the zebrafish heart requires directed migration of cardiac progenitor cells. Developmental Cell   14(2):287-297 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Prim-15
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 20-25 somites to Prim-15

Fig. 5 Leftward Displacement of CPCs and Cardiac Rotation Are Affected upon Reduced or Ectopic BMP Signaling (A–C) Selected images of confocal time-lapse recordings of laf/alk8 (A) heat-shock induced (at 15-somite stage) tg(hsp70:noggin3) (B) and tg(hsp70:bmp2b) (C) embryos with individual cells marked and tracked over a 200 min period. (D) Quantification of clockwise cardiac cone rotation in wild-type laf/alk8 mutant tg(hsp70:noggin3) and tg(hsp70:bmp2b) embryos (mean ± SEM). (E) ISH with a cmlc2 riboprobe on tg(hsp70:bmp2b) embryos heat shocked at various time points (indicated at the top). Arrows and arrowheads indicate CPCs not properly incorporated into the cardiac tube. Heat shock at 15-somite stage, no cardiac jogging (n = 14/16); 18-somite stage, no cardiac jogging (n = 12/20); 22-somite stage, leftward jogging (n = 17/19).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myl7 fr13Tg; twu34Tg heat shock Prim-15 heart tube ISH
fr13Tg; twu34Tg heat shock Prim-15 heart tube ISH
fr13Tg; twu34Tg heat shock Prim-15 heart tube ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
acvr1ltm110b/tm110b standard conditions 20-25 somites - Prim-5 embryonic heart tube development disrupted, abnormal
20-25 somites - Prim-5 heart rudiment rotated, abnormal
fr13Tg heat shock 20-25 somites - Prim-5 embryonic heart tube development disrupted, abnormal
20-25 somites - Prim-5 heart rudiment rotated, abnormal
fr13Tg; twu34Tg heat shock Prim-15 cardiac muscle cell mislocalised, abnormal
Prim-15 heart looping disrupted, abnormal
Prim-15 heart tube morphology, abnormal
fr13Tg; twu34Tg heat shock Prim-15 heart looping disrupted, abnormal
fr13Tg; twu34Tg heat shock Prim-15 heart looping process quality, normal
fr14Tg heat shock 20-25 somites - Prim-5 embryonic heart tube development disrupted, abnormal
20-25 somites - Prim-5 heart rudiment rotated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 14(2), Smith, K.A., Chocron, S., von der Hardt, S., de Pater, E., Soufan, A., Bussmann, J., Schulte-Merker, S., Hammerschmidt, M., and Bakkers, J., Rotation and asymmetric development of the zebrafish heart requires directed migration of cardiac progenitor cells, 287-297, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell