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ZFIN ID: ZDB-FIG-080325-57
Cheng et al., 2008 - Zebrafish cdx1b regulates expression of downstream factors of Nodal signaling during early endoderm formation. Development (Cambridge, England)   135(5):941-952 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 30%-epiboly to Long-pec

Fig. S4 Analyses of relationships between cdx1b and components of Nodal signaling. A slightly increased expression area of cdx1b in the intestines was detected in a 48-hpf squintcz35 mutant embryo (n=9) (B) when compared with a sibling wild-type embryo (A). However, a subsequent semiquantitative RT-PCR revealed no significant differences when comparing cdx1b expression levels in 48-hpf squintcz35 homozygous mutants with their sibling wild-type or AB wild-type embryos. No differences in cdx1b expression was observed in 48-hpf cycb16 (n=7) (D) and 4-6s oepm134 mutants (n=26) (F) compared with sibling wild-type embryos (C,E). Respective expression levels of cyclops (n=54) and oep (n=54) in 85% epiboly cdx1b morphants (H,J) were not altered compared with wild-type embryos (G,I). Similar expression levels of sqt (n=172) and bon (n=91) were detected in 30% (L) and 40% (N) G,I). Similar expression levepiboly morphants when compared with respective wild-type embryos (K,M

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdx1b WT standard conditions 1-4 somites to 5-9 somites ectoderm ISH
1-4 somites to 5-9 somites endoderm ISH
1-4 somites to 5-9 somites mesoderm ISH
Long-pec gut ISH
Long-pec intestine ISH
ndr1cz35/cz35 standard conditions Long-pec gut ISH
Long-pec intestine ISH
ndr2Df(Chr12:hhex)b16/b16 standard conditions Long-pec gut ISH
Long-pec intestine ISH
tdgf1m134/m134 standard conditions 1-4 somites to 5-9 somites ectoderm ISH
1-4 somites to 5-9 somites endoderm ISH
1-4 somites to 5-9 somites mesoderm ISH
mixl1 WT standard conditions 30%-epiboly margin ISH
WT + MO1-cdx1b standard conditions 30%-epiboly margin ISH
ndr1 WT standard conditions 30%-epiboly margin ISH
WT + MO1-cdx1b standard conditions 30%-epiboly margin ISH
ndr2 WT standard conditions 75%-epiboly hypoblast ISH
WT + MO1-cdx1b standard conditions 75%-epiboly hypoblast ISH
tdgf1 WT standard conditions 75%-epiboly hypoblast ISH
WT + MO1-cdx1b standard conditions 75%-epiboly hypoblast ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development