Analyses of relationships between cdx1b and downstream factors of Nodal signaling in zebrafish. Reductions in respective gata5-, cas-, sox17- and foxa2-expressing endodermal cell numbers were observed in 85% epiboly morphants (C,G,H,K,L,O) compared with respective cdx1b-4mm-MO-injected (A,E,F,I,J,M) and wild-type (B,N) embryos. Embryos co-injected with either cdx1b mRNA or protein showed restoration of respective gata5- (D) and foxa2-expressing (P) endodermal cell numbers. Percentages of morphants showing decreases in the respective numbers of gata5-, cas-, sox17- and foxa2-expressing endodermal cells (Q). Comparison of the respective gata5-, cas-, sox17- and foxa2-expressing endodermal cell numbers in morphants (yellow bars), and wild type (orange bars) and cdx1b-4mm-MO-injected (brown bars) embryos (n=10 each) (R). Comparison of percentages of embryos showing decreased foxa2- or gata5-expressing endodermal cell numbers in respective embryos injected with either cdx1b MO (brown bars), cdx1b MO and cdx1b mRNA (orange bars), or cdx1b MO and different amounts of Cdx1b protein (1/40x, yellow bars; 1/2x, green bars) (S). Comparison of the respective foxa2- and gata5-expressing endodermal cell numbers in morphants (brown bars) and embryos co-injected with cdx1b MO and either cdx1b mRNA (orange bars) or protein (yellow bars) (T). Error bars represent standard error. Dfc, dorsal forerunner cell; wt, wild type.
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