FIGURE

Fig. 2

ID

ZDB-FIG-080325-46

Publication

Cheng et al., 2008 - Zebrafish cdx1b regulates expression of downstream factors of Nodal signaling during early endoderm formation

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Fig. 2

cdx1b antisense MO knockdown analyses. cdx1b 24 hpf (A-F) and 48 hpf (M,N) zebrafish morphants; cdx1b-4mm-MO-injected 24 hpf (G-I) and 48 hpf (O,P) embryos; wild-type 24 hpf (J-L) and 48 hpf (Q,R) embryos. Green fluorescence was not detected in cdx1b MO and CMV-cdx1b-mo-GFP co-injected (T) 27 hpf embryos, whereas bright green fluorescence was detected in cdx1b-4mm MO and CMV-cdx1b-mo-GFP co-injected embryos (S). The expression patterns of shh were compared in 28 hpf wild type (U,V) and morphants (W,X). Scale bars: 100 μm. a, atrium; bp, basal plate midbrain; fp, floor plate; hy, hypothalamus; v, ventricle; wt, wild type; zli, zona limitans intrathalamica.

Expression Data

Gene:	shha
Fish:	WT WT + MO1-cdx1b
Knockdown Reagent:	MO1-cdx1b
Anatomical Terms:	basal plate midbrain region floor plate hypothalamus zona limitans intrathalamica
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-cdx1b
Knockdown Reagent:	MO1-cdx1b
Observed In:	basal plate midbrain region eye heart looping hypothalamus pericardium trunk zona limitans intrathalamica
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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