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ZFIN ID: ZDB-FIG-080114-42
Yeh et al., 2008 - AML1-ETO reprograms hematopoietic cell fate by downregulating scl expression. Development (Cambridge, England)   135(2):401-410 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-15

Fig. S6 AML1-ETO suppresses the erythroid and monocytic cell fates, and promotes the granulocytic cell fate in chordin morphants. (A) In situ hybridization of gata1, mpo and l-plastin in wild-type and AML1-ETO transgenic embryos. All of the embryos have been injected with 50 mM chordin antisense morpholino oligonucleotides (Open Biosystems) and subjected to the same heat treatment. The expanded pool of blood cells in the chordin morphants expresses gata1 and l-plastin (A, upper panels), whereas induced expression of AML1-ETO downregulates both gata1 and l-plastin but upregulates mpo (A, lower panels). The embryos were at 22 hpf for gata1 staining and 31 hpf for mpo and l-plastin staining. (B,C) Cross sections of chordin morphants stained with l-plastin probes. While l-plastin+ cells are often seen in the wild-type embryos, these cells are not seen in the AML1-ETO transgenic embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gata1a WT + MO1-chrd heat shock 20-25 somites megakaryocyte erythroid progenitor cell ISH
f3Tg + MO1-chrd heat shock 20-25 somites megakaryocyte erythroid progenitor cell ISH
lcp1 WT + MO1-chrd heat shock Prim-15 monocyte ISH
f3Tg + MO1-chrd heat shock Prim-15 granulocyte ISH
mpx WT + MO1-chrd heat shock Prim-15 Not Detected granulocyte ISH
f3Tg + MO1-chrd heat shock Prim-15 granulocyte ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development