ZFIN ID: ZDB-FIG-080114-23
Schneider et al., 2008 - Calcium fluxes in dorsal forerunner cells antagonize {beta}-catenin and alter left-right patterning. Development (Cambridge, England)   135(1):75-84 Full text @ Development
Knockdown Reagent:
Anatomical Terms:
Stage Range: 75%-epiboly to 20-25 somites

Fig. 5 Ca2+ inhibition activates β-catenin and β-catenin is sufficient to alter laterality. Confocal images of β-catenin immunolocalization at 70-80% epiboly in (A) wt and (B) thapsigargin-treated embryos. HRP staining of β-catenin protein oriented on the DFC region in (C) wt, (D) thapsigargin-treated and (E) DFC-targeted Axin-1 MO-injected embryos; arrowheads indicate β-catenin-positive nuclei. (F) Comparison of total nuclear β-catenin in wt and thapsigargin-treated embryos.* indicates P-value of 2.5x10-5. (G) TopFlash (top) vs. FopFlash (fop) luciferase reporter constructs analyzed for relative luminescence in wt or thapsigargin-treated embryos, normalized to control (Renilla) luciferase. Data are means ± s.e. (H,I) Combined lefty1 and lefty2 expression (arrows) in the left LPM and brain in wt (H) and bilateral (I) in DFC-targeted β-catenin RNA-injected embryos: dorsal-anterior view, 19-23 somites.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctnnb1 WT standard conditions 75%-epiboly EVL IHC
75%-epiboly yolk syncytial layer IHC
WT chemical treatment: thapsigargin 75%-epiboly EVL IHC
75%-epiboly forerunner cell group IHC
75%-epiboly yolk syncytial layer IHC
WT + MO2-axin1 standard conditions 75%-epiboly forerunner cell group IHC
lft1 WT standard conditions 20-25 somites lateral plate mesoderm ISH
lft2 WT standard conditions 20-25 somites lateral plate mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development