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ZFIN ID: ZDB-FIG-080114-23
Schneider et al., 2008 - Calcium fluxes in dorsal forerunner cells antagonize {beta}-catenin and alter left-right patterning. Development (Cambridge, England)   135(1):75-84 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 75%-epiboly to 20-25 somites

Fig. 5 Ca2+ inhibition activates β-catenin and β-catenin is sufficient to alter laterality. Confocal images of β-catenin immunolocalization at 70-80% epiboly in (A) wt and (B) thapsigargin-treated embryos. HRP staining of β-catenin protein oriented on the DFC region in (C) wt, (D) thapsigargin-treated and (E) DFC-targeted Axin-1 MO-injected embryos; arrowheads indicate β-catenin-positive nuclei. (F) Comparison of total nuclear β-catenin in wt and thapsigargin-treated embryos.* indicates P-value of 2.5x10-5. (G) TopFlash (top) vs. FopFlash (fop) luciferase reporter constructs analyzed for relative luminescence in wt or thapsigargin-treated embryos, normalized to control (Renilla) luciferase. Data are means ± s.e. (H,I) Combined lefty1 and lefty2 expression (arrows) in the left LPM and brain in wt (H) and bilateral (I) in DFC-targeted β-catenin RNA-injected embryos: dorsal-anterior view, 19-23 somites.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctnnb1 WT standard conditions 75%-epiboly EVL IHC
75%-epiboly yolk syncytial layer IHC
WT chemical treatment: thapsigargin 75%-epiboly EVL IHC
75%-epiboly forerunner cell group IHC
75%-epiboly yolk syncytial layer IHC
WT + MO2-axin1 standard conditions 75%-epiboly forerunner cell group IHC
lft1 WT standard conditions 20-25 somites lateral plate mesoderm ISH
lft2 WT standard conditions 20-25 somites lateral plate mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development