FIGURE

Fig. 5

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ZDB-FIG-071204-29

Publication

Li et al., 2007 - Redundant activities of Tfap2a and Tfap2c are required for neural crest induction and development of other non-neural ectoderm derivatives in zebrafish embryos

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Fig. 5

Gene expression characteristic of neural crest is absent, but of the neural plate border and pre-placodal region is present in tfap2a/c deficient embryos. (A, B) Lateral views of 22 hpf embryos processed to reveal sox10 expression, which is present in migratory neural crest streams (arrowheads) and the otic vesicle (ot) in panel A, a control embryo; in panel B, a tfap2a^-/c^MO embryo, it is only present in the ot (11 of 11 tfap2a^-/c^MO embryos). (C, D) Dorsal views of 24 hpf embryos processed to reveal dlx2 expression, which is present in cranial migratory neural crest streams (arrowheads) and brain in panel C, a control embryo, but only in the latter in panel D, a tfap2a/c deficient embryo (10 of 10 tfap2a^-/c^MO embryos). (E–N) Dorsal views of flat-mounted, 11 hpf embryos processed to reveal the indicated markers. foxd3 and snai1b expression that are characteristic of premigratory neural crest (pnc) cells are absent in embryos injected with tfap2a e2i2 MO and tfap2c e3i3 MO (hereafter, tfap2a^MO/c^MO embryo) (foxd3, 27 of 27 tfap2a^MO/c ^MO embryos; snai1b, 29 of 29 tfap2a^MO/c^MO embryos. Also not shown, at the same stage, sox10, 29 of 29 tfap2a^MO/c^MO embryos). Expression of pax3, msxb and dlx3b expression appear normal in tfap2a^MO/c^MO embryos (100%, n is greater or equal to 30 tfap2a^MO/c^MO embryos for each gene, also not shown, zic2b). Scale bars: 100 μm.

Expression Data

Genes:	dlx2a dlx3b foxd3 msx1b pax3a snai1b sox10
Fish:	WT tfap2a^ts213/ts213 + MO2-tfap2c WT + MO2-tfap2c + MO4-tfap2a
Knockdown Reagents:	MO2-tfap2c MO4-tfap2a
Anatomical Terms:	brain neural crest neural plate otic vesicle
Stage Range:	1-4 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 304(1), Li, W., and Cornell, R.A., Redundant activities of Tfap2a and Tfap2c are required for neural crest induction and development of other non-neural ectoderm derivatives in zebrafish embryos, 338-354, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.