FIGURE

Fig. 5

ID

ZDB-FIG-070925-21

Publication

Varga et al., 2007 - Chordin expression, mediated by Nodal and FGF signaling, is restricted by redundant function of two beta-catenins in the zebrafish embryo

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Fig. 5

Germ-ring expression of chordin is repressed by Wnt8 through the transcriptional repressors Vox and Vent. ich embryos co-injected with MOs targeted against both ORFs of wnt8 (B) or with MOs against both vox and vent (D) show ectopic expression of chd around the germ-ring. Uninjected ich embryos do not express chd (A and C). Suppression of β-catenin signaling in the germ-ring of ich embryos by injection of βcat1MO + βcat2MO (MO1 + MO2) causes a decrease of vox and vent expression (compare E with F, and G with H). Embryos are shown in lateral (A–D) or animal (E–H) views at 70% epiboly. Concentrations of MOs injected were 0.6 mM of each of the two Wnt8MOs, 0.7 mM of each of voxMO and ventMO, and 3 mM of each of the two β-catenin MOs.

Expression Data

Genes:	chrd vent vox
Fish:	brs^b2; ctnnb2^p1/p1 brs^b2; ctnnb2^p1/p1 + MO1-ctnnb2 + MO2-ctnnb1 brs^b2; ctnnb2^p1/p1 + MO1-wnt8a + MO2-wnt8a brs^b2; ctnnb2^p1/p1 + MO1-vent + MO1-vox
Knockdown Reagents:	MO1-ctnnb2 MO1-vent MO1-vox MO1-wnt8a MO2-ctnnb1 MO2-wnt8a
Anatomical Terms:	germ ring whole organism
Stage:	Shield

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 124(9-10), Varga, M., Maegawa, S., Bellipanni, G., and Weinberg, E.S., Chordin expression, mediated by Nodal and FGF signaling, is restricted by redundant function of two beta-catenins in the zebrafish embryo, 775-791, Copyright (2007) with permission from Elsevier. Full text @ Mech. Dev.