FIGURE

Fig. S3

ID

ZDB-FIG-070802-56

Publication

Gansner et al., 2007 - Essential role of lysyl oxidases in notochord development

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Fig. S3

Lysyl oxidase and collagen II splice morpholinos decrease the abundance of wild-type splice forms and cause specific changes in splice site usage. (A) loxl1, (B) loxl2b, (C) loxl3b, (D) loxl5b and (E) col2a1. Wild-type embryos injected with the indicated doses of morpholino were harvested at 20 hpf and cDNA prepared from equal amounts of RNA. The intensity of the wild-type (WT) product obtained by PCR was quantified and the percentage of wild-type splice form remaining after morpholino knockdown determined, as noted. This measure is independent of processes that may depress levels of the mutant (Mut) splice products, including degradation through nonsense-mediated decay. The primer set used to amplify loxl1 does not amplify the mutant band.

Expression Data

Genes:	col2a1a loxl1 loxl2b loxl3b loxl5b
Fish:	WT WT + MO1-col2a1a WT + MO1-loxl1 WT + MO1-loxl2b WT + MO1-loxl3b WT + MO1-loxl5b
Knockdown Reagents:	MO1-col2a1a MO1-loxl1 MO1-loxl2b MO1-loxl3b MO1-loxl5b
Anatomical Term:	whole organism
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 307(2), Gansner, J.M., Mendelsohn, B.A., Hultman, K.A., Johnson, S.L., and Gitlin, J.D., Essential role of lysyl oxidases in notochord development, 202-213, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.