Fig. 5

Morpholino knockdown of loxl1 or loxl1 and loxl5b together results in notochord distortion. Wild-type embryos were injected with morpholinos (MO) to notochord-expressed lysyl oxidases and photographed at 24 hpf. (A) 12 ng standard control; (B) 6 ng loxl1; (C) 3.7 ng loxl2b; (D) 12 ng loxl3b; (E) 7.4 ng loxl5b; (F) 2.4 ng loxl1 and 5 ng loxl5b. The notochord (arrowheads) is strikingly distorted after knockdown of loxl1 (B), and caudal vein edema develops with the loxl5b morpholino (E, arrows). Combining doses of loxl1 and loxl5b morpholino that do not cause notochord distortion alone also recapitulates the notochord phenotype seen with neocuproine and β-aminopropionitrile treatment (F). This distortion is specifically rescued by co-injection of mRNA encoding either loxl1 (H) or loxl5b (I), but not control sequence (G).