FIGURE

Fig. S1

ID

ZDB-FIG-070802-55

Publication

Gansner et al., 2007 - Essential role of lysyl oxidases in notochord development

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Fig. S1

Comparison of the notochord distortion obtained in zebrafish using three distinct methods of lysyl oxidase inhibition at 24 hpf. Wild-type embryos were incubated in 10 μM neocuproine (A), 10 mM ®-aminopropionitrile supplemented with 10 μM CuCl2 (B), 100 mM ®-aminopropionitrile supplemented with 10 μM CuCl2 (C), or injected with 6 ng each of loxl1 and loxl5b start (ATG) morpholino (D). 10 μM neocuproine (A) causes greater notochord distortion than 10 mM ®-aminopropionitrile (B), but an identical phenotype is obtained with 100 mM ®-aminopropionitrile (A vs. C). Melanin pigmentation has not yet developed in the embryos pictured, but is not affected by ®-aminopropionitrile treatment or by the morpholino injection.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-loxl5b + MO3-loxl1
Knockdown Reagents:	MO2-loxl5b MO3-loxl1
Observed In:	notochord
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 307(2), Gansner, J.M., Mendelsohn, B.A., Hultman, K.A., Johnson, S.L., and Gitlin, J.D., Essential role of lysyl oxidases in notochord development, 202-213, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.