ZFIN ID: ZDB-FIG-070219-11
Hernandez et al., 2007 - Cyp26 enzymes generate the retinoic acid response pattern necessary for hindbrain development. Development (Cambridge, England)   134(1):177-187 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 5-9 somites to 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 14-19 somites

Fig. 2 cyp26b1 and cyp26c1 function redundantly with cyp26a1 to pattern the hindbrain. Whole-mount RNA in situ hybridizations at 18 hpf (A-J) and 13 hpf (K,L) and immunostaining at 48 hpf (M,N) in wild-type (left column) and cyp26a1-/- (right column) embryos injected with MOs as shown on the left. (A-H) pax2a (blue) marks the optic stalk (os), posterior midbrain and cerebellum (bracket), and the otic vesicles (ov); whereas hoxd4 (also blue) marks the r7-r8 territory and krox20 (red) marks r3 and r5. MO depletion of Cyp26b1 and/or Cyp26c1 does not affect this pattern in wild-type embryos (C,E,G), but progressively posteriorizes the hindbrain in cyp26a1-/- embryos (D,F,H). Arrowhead marks the r6-r7 boundary, which is shifted to the anterior hindbrain in Cyp26-depleted embryos. (I,J) en3 (red) marks the posterior midbrain and cerebellum (bracket) and hoxb1a (blue) marks r4, which is shifted anteriorly in Cyp26-depleted embryos. (K,L) pax2a (blue) and krox20 (red) are expressed as described above. vhnf1 (also blue) is expressed in the posterior hindbrain up to the r5-r6 boundary (arrowheads) and is also shifted anteriorly in Cyp26-depleted embryos. (M,N) The isl1-GFP transgene (green) marks cranial motor neurons (nV: trigeminal motor neurons in r2 and r3; nVII: facial motor neurons in r4-6; nX: vagal motor neurons in r8) whereas the zn5 antibody (red) marks spinal motor neurons (arrows), pharyngeal arch endoderm (pe, arrowheads mark individual pharyngeal arches) and other structures. The large white arrowhead indicates the mid-hindbrain boundary. In Cyp26-depleted embryos, the motor neurons of the vagus nerve (nX) are expanded anteriorly, as are the spinal motor neurons. Scale bars: 100 μm. Scale bar in A is for A-H,K,L; scale bar in I is for I,J.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
egr2b cyp26a1rw716/rw716 + MO1-cyp26b1 + MO1-cyp26c1 standard conditions 5-9 somites Not Detected presumptive rhombomere 3 ISH
5-9 somites Not Detected presumptive rhombomere 5 ISH
14-19 somites Not Detected rhombomere 3 ISH
14-19 somites Not Detected rhombomere 5 ISH
cyp26a1rw716/rw716 + MO1-cyp26b1 + MO1-dnd1 standard conditions 14-19 somites rhombomere 3 ISH
14-19 somites rhombomere 5 ISH
cyp26a1rw716/rw716 + MO1-cyp26c1 + MO1-dnd1 standard conditions 14-19 somites rhombomere 5 ISH
cyp26a1rw716/rw716 + MO1-dnd1 standard conditions 14-19 somites rhombomere 3 ISH
14-19 somites rhombomere 5 ISH
en2b cyp26a1rw716/rw716 + MO1-cyp26b1 + MO1-cyp26c1 standard conditions 14-19 somites midbrain ISH
14-19 somites midbrain hindbrain boundary ISH
14-19 somites rhombic lip ISH
hnf1ba cyp26a1rw716/rw716 + MO1-cyp26b1 + MO1-cyp26c1 standard conditions 5-9 somites presumptive rhombomere 6 ISH
5-9 somites presumptive rhombomere 7 ISH
5-9 somites presumptive rhombomere 8 ISH
hoxb1a cyp26a1rw716/rw716 + MO1-cyp26b1 + MO1-cyp26c1 standard conditions 14-19 somites rhombomere 4 ISH
hoxd4a cyp26a1rw716/rw716 + MO1-cyp26b1 + MO1-cyp26c1 standard conditions 14-19 somites rhombomere 7 ISH
14-19 somites rhombomere 8 ISH
cyp26a1rw716/rw716 + MO1-cyp26b1 + MO1-dnd1 standard conditions 14-19 somites rhombomere 7 ISH
14-19 somites rhombomere 8 ISH
cyp26a1rw716/rw716 + MO1-cyp26c1 + MO1-dnd1 standard conditions 14-19 somites rhombomere 7 ISH
14-19 somites rhombomere 8 ISH
cyp26a1rw716/rw716 + MO1-dnd1 standard conditions 14-19 somites rhombomere 7 ISH
14-19 somites rhombomere 8 ISH
pax2a cyp26a1rw716/rw716 + MO1-cyp26b1 + MO1-cyp26c1 standard conditions 5-9 somites hindbrain neural keel ISH
5-9 somites midbrain hindbrain boundary neural rod ISH
5-9 somites midbrain neural rod ISH
5-9 somites optic vesicle ISH
14-19 somites midbrain ISH
14-19 somites midbrain hindbrain boundary ISH
14-19 somites rhombic lip ISH
cyp26a1rw716/rw716 + MO1-cyp26b1 + MO1-dnd1 standard conditions 14-19 somites midbrain ISH
14-19 somites midbrain hindbrain boundary ISH
14-19 somites otic vesicle ISH
14-19 somites rhombic lip ISH
cyp26a1rw716/rw716 + MO1-cyp26c1 + MO1-dnd1 standard conditions 14-19 somites midbrain ISH
14-19 somites midbrain hindbrain boundary ISH
14-19 somites otic vesicle ISH
14-19 somites rhombic lip ISH
cyp26a1rw716/rw716 + MO1-dnd1 standard conditions 14-19 somites midbrain ISH
14-19 somites midbrain hindbrain boundary ISH
14-19 somites optic stalk ISH
14-19 somites otic vesicle ISH
14-19 somites rhombic lip ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cyp26a1rw716/rw716 + MO1-cyp26b1 + MO1-cyp26c1 standard conditions 14-19 somites hindbrain wholly posterioralized, abnormal
14-19 somites pharyngeal endoderm condensed, abnormal
14-19 somites rhombomere 4 increased size, abnormal
14-19 somites vagal lobe distended, abnormal
cyp26a1rw716/rw716 + MO1-cyp26b1 + MO1-dnd1 standard conditions 14-19 somites rhombomere 4 increased size, abnormal
cyp26a1rw716/rw716 + MO1-cyp26c1 + MO1-dnd1 standard conditions 14-19 somites hindbrain morphology, abnormal
cyp26a1rw716/rw716 + MO1-dnd1 standard conditions 14-19 somites rhombomere 4 increased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development