ZFIN ID: ZDB-FIG-070219-18
Hernandez et al., 2007 - Cyp26 enzymes generate the retinoic acid response pattern necessary for hindbrain development. Development (Cambridge, England)   134(1):177-187 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 14-19 somites

Fig. S4 cyp26 genes function redundantly to protect the anterior hindbrain from endogenous RA. cyp26a1 and cyp26c1 are responsible for setting the r3-r4 limit. (A,C,E,G) Knocking-down cyp26b1 and/or c1 has no effect on hindbrain patterning in wild-type embryos. (B) cyp26a1 mutants exhibit a mild expansion of r4. This effect is enhanced by knocking-down cyp26b1 (D), but hoxb1a expression is shifted to the presumptive cerebellum (En-expressing domains) by knocking-down cyp26c1 (F). Embryos depleted for all three cyp26 proteins exhibit a profound anterior shift of hoxb1 expression to the mid-hindbrain domain (H). Scale bar: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
en2b cyp26a1rw716/rw716 + MO1-cyp26b1 + MO1-cyp26c1 standard conditions 14-19 somites midbrain ISH
14-19 somites midbrain hindbrain boundary ISH
14-19 somites rhombic lip ISH
cyp26a1rw716/rw716 + MO1-cyp26b1 + MO1-dnd1 standard conditions 14-19 somites midbrain ISH
14-19 somites midbrain hindbrain boundary ISH
14-19 somites rhombic lip ISH
cyp26a1rw716/rw716 + MO1-cyp26c1 + MO1-dnd1 standard conditions 14-19 somites midbrain ISH
14-19 somites midbrain hindbrain boundary ISH
14-19 somites rhombic lip ISH
cyp26a1rw716/rw716 + MO1-dnd1 standard conditions 14-19 somites midbrain ISH
14-19 somites midbrain hindbrain boundary ISH
14-19 somites rhombic lip ISH
hoxb1a cyp26a1rw716/rw716 + MO1-cyp26b1 + MO1-cyp26c1 standard conditions 14-19 somites midbrain ISH
cyp26a1rw716/rw716 + MO1-cyp26b1 + MO1-dnd1 standard conditions 14-19 somites rhombomere 4 ISH
cyp26a1rw716/rw716 + MO1-cyp26c1 + MO1-dnd1 standard conditions 14-19 somites rhombic lip ISH
cyp26a1rw716/rw716 + MO1-dnd1 standard conditions 14-19 somites rhombomere 4 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cyp26a1rw716/rw716 + MO1-cyp26b1 + MO1-cyp26c1 standard conditions 14-19 somites hindbrain wholly posterioralized, abnormal
14-19 somites rhombomere 4 increased size, abnormal
cyp26a1rw716/rw716 + MO1-cyp26b1 + MO1-dnd1 standard conditions 14-19 somites rhombomere 4 increased size, abnormal
cyp26a1rw716/rw716 + MO1-cyp26c1 + MO1-dnd1 standard conditions 14-19 somites hindbrain wholly posterioralized, abnormal
14-19 somites rhombomere 4 increased size, abnormal
cyp26a1rw716/rw716 + MO1-dnd1 standard conditions 14-19 somites rhombomere 4 increased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development