Wound Response/Inflammatory Factors, Secreted Molecules, and MMPs Are Upregulated during Zebrafish Heart Regeneration (A) Genes encoding wound response and inflammatory factors, secreted molecules, and MMPs were clustered based on their expression patterns at 3, 7, and 14 dpa. The gene name, gene symbol, and fold change of each gene can be found in Dataset S1. The color chart indicates fold change of expression, and red and green represent increased and decreased expression, respectively. (B) General trend and average fold change of expression of wound response/inflammatory genes, secreted molecules, and MMPs. The wound response/inflammatory genes are expressed early (peak at 3 dpa) during zebrafish heart regeneration. Genes coding for secreted molecules begin to express at 3 dpa and the expression level reach a peak at 7 dpa. The MMPs start to express at 7 dpa and last until 14 dpa. (C) Semiquantitative RT-PCR of a subset of genes coding for secreted molecules, including apoEb (apolipoprotein Eb), vegfc (vascular endothelial growth factor c), granulin A, pdgf-a (platelet-derived growth factor-a; labeled in red), thymosin β4, midkine a, and cxcl12a (chemokine (c-x-c motif) ligand 12a). β-actin was used as a loading control. The RT-PCR results confirmed the temporal expression patterns of these genes as determined by microarray analysis.
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